Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)

Product name

Anti-MCM2 antibody [CRCT2.1 (D1.9H5)]
See all MCM2 primary antibodies
Description

Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2
Host species

Mouse
Specificity

Recognises human Mcm2
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein corresponding to MCM2. Bacterially expressed human Mcm2.
Positive control
- Tonsil, any tissue with high proliferative index
General notes

Clone Number: CRCT2.1 (D1.9H5)

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
Purification notes

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Clonality

Monoclonal
Clone number

CRCT2.1 (D1.9H5)
Myeloma

Sp2
Isotype

IgG1
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Our Abpromise guarantee covers the use of ab6153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB		Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similarities

Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Cellular localization

Nucleus.
Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
- CDC like 1 antibody
- cdc19 antibody
- CDCL 1 antibody
- CDCL1 antibody
- Cell devision cycle like 1 antibody
- Cyclin like 1 antibody
- D3S3194 antibody
- DNA replication licensing factor MCM2 antibody
- KIAA0030 antibody
- MCM 2 antibody
- MCM2 antibody
- MCM2 minichromosome maintenance deficient 2 mitotin antibody
- MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
- MCM2 minichromosome maintenance deficient 2, mitotin antibody
- MCM2_HUMAN antibody
- MGC10606 antibody
- Minichromosome maintenance complex component 2 antibody
- Minichromosome maintenance deficient 2 (mitotin) antibody
- Minichromosome maintenance deficient 2 mitotin antibody
- Minichromosome maintenance protein 2 antibody
- Minichromosome maintenance protein 2 homolog antibody
- Mitotin antibody
- Nuclear protein BM28 antibody
- OTTHUMP00000216047 antibody
- OTTHUMP00000216050 antibody
see all

Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)

Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.

Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.

Western blot protocols
Immunohistochemistry protocols

This product has been referenced in:

Scapulatempo-Neto C et al. Characterization of topoisomerase II a and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13:1891-1898 (2017). IHC-P ; Human . Read more (PubMed: 28454340) »
Zhang AT et al. Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication. J Cell Sci 124:2058-69 (2011). Read more (PubMed: 21610089) »

See all 8 Publications for this product

Publishing research using ab6153? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

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1-10 of 10 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Kidney biopsies)

Specification

Kidney biopsies

Fixative

Paraformaldehyde

Permeabilization

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Stomach)

Specification

Stomach

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate Buffer

Permeabilization

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Mar 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293T)

Loading amount

20 µg

Specification

HEK293T

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Blocking step

Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Liver biopsy)

Specification

Liver biopsy

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer

Permeabilization

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Saira Khalid

Verified customer

Submitted Apr 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - other (HeLa)

Loading amount

100 µg

Specification

HeLa

Gel Running Conditions

Reduced Denaturing (8% gel)

Blocking step

Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 18 2008

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - nuclear (SupT1, Jurkat, Hela cells)

Loading amount

15 µg

Specification

SupT1, Jurkat, Hela cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Nov 08 2006

Question

A customer needs an ab to human MCM2 for IHC and IF. The closest I found is ab6153, but it was not tested for IF. Would you expect it to be applicable for IF? (We know that the abpromise will not be valid for IF).

Abcam community

Verified customer

Asked on Jul 16 2006

Answer

The antibody has good chances of working in other types of immunohistochemistry other than those already tested as it has the ability to recognise the native form of the protein. If the customer means IF as in ICC/IF I would recommend to try fixing the cells in PFA 5-10min. If the customer means IF as in IHC-P or IHC-Fr with an IF detection method I would recommend to follow our recommendation for those methods but use an IF amplification kit (i.e either a biotin secondary + extra-avidin/FITC or TRITC method or more sensitive methods which come from Kits eg the TSA kit). I hope this will help your customer,

Abcam Scientific Support

Answered on Jul 17 2006

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - nuclear (HeLa)

Specification

HeLa

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Sep 21 2005

Question

I am going to buy your products, MCM2 antibody. But I found several same antibody from your company. This antibody will be used in Western blot. Could you tell me what difference between them(such as, ab1896, ab6153,ab4461)? Are they all antibody for phosphoMCM2? Could you help me to decide which I had better to choose? Many thanks

Abcam community

Verified customer

Asked on Nov 04 2004

Answer

All the information available for this product is listed on the on-line datasheet (price, datasheet, immunogen, publication, suitability, cross-reactivity). I suggest you take a look at the datasheets for these particular products in order to ascertain their level of usefulness in your experimental procedures. I'm sorry for not being able to give you more guidance. If you require additional information to that provided on the product datasheet, please contact us again and we will gladly assist you.

Abcam Scientific Support

Answered on Nov 05 2004

Question

I need to know it's success rate for immunhistochemistry on formalin fixed, paraffin embedded tissues.

Abcam community

Verified customer

Asked on Jan 14 2002

Answer

All the information we have for this product for use in IHC is on the datasheet, including recommended dilutions and advice for antigen retrieval. I also suggest that you click through to the review submitted by a researcher who successfully used this antibody in IHC on human lung cancer paraffin embedded tissue. In terms of the "success rate" this will depend on a number variables, including the species that you are studying. Species cross-reactivity for this product has not been tested. However, as discussed this antibody works very well in IHC on human tissue.

Abcam Scientific Support

Answered on Jan 15 2002

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]