Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)
![Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)](https://www.abcam.com/ps/products/6/ab6153/Images/ab6153-12068-anti-mcm2-antibody-crct21-d19h5-western-blot.jpg "Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)")
Key features and details
- Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MCM2 antibody [CRCT2.1 (D1.9H5)]
See all MCM2 primary antibodies -
Description
Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to MCM2. Bacterially expressed human Mcm2.
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Positive control
- Tonsil, any tissue with high proliferative index
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
CRCT2.1 (D1.9H5) -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).
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| Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
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Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
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Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab6153 has been referenced in 10 publications.
- Wienert B et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun 11:2109 (2020). PubMed: 32355159
- Mishra S et al. Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications. Cell 174:803-817.e16 (2018). PubMed: 30057114
- Scapulatempo-Neto C et al. Characterization of topoisomerase II a and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13:1891-1898 (2017). IHC-P ; Human . PubMed: 28454340
- Zhang AT et al. Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication. J Cell Sci 124:2058-69 (2011). PubMed: 21610089
- Shtutman M et al. Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing. Proc Natl Acad Sci U S A 107:7377-82 (2010). WB ; Human . PubMed: 20368428
- Sarfraz S et al. Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection. BMC Microbiol 8:133 (2008). IHC-P ; Human . PubMed: 18680610
- Villadsen R et al. Evidence for a stem cell hierarchy in the adult human breast. J Cell Biol 177:87-101 (2007). PubMed: 17420292
- Niklaus AL et al. Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women. Hum Reprod 22:1778-88 (2007). PubMed: 17371803
- Canela N et al. Identification of an hexapeptide that binds to a surface pocket in cyclin A and inhibits the catalytic activity of the complex cyclin-dependent kinase 2-cyclin A. J Biol Chem 281:35942-53 (2006). WB ; Human . PubMed: 17001081
- Duursma A & Agami R p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation. Mol Cell Biol 25:6937-47 (2005). WB . PubMed: 16055707