Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)
![Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)](https://www.abcam.com/ps/products/6/ab6153/Images/ab6153-12068-anti-mcm2-antibody-crct21-d19h5-western-blot.jpg "Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)")
Key features and details
- Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MCM2 antibody [CRCT2.1 (D1.9H5)]
See all MCM2 primary antibodies -
Description
Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2 -
Host species
Mouse -
Specificity
Recognises human Mcm2 -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to MCM2. Bacterially expressed human Mcm2.
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Positive control
- Tonsil, any tissue with high proliferative index
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General notes
Clone Number: CRCT2.1 (D1.9H5)The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
CRCT2.1 (D1.9H5) -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
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Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
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Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab6153 has been referenced in 10 publications.
- Wienert B et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun 11:2109 (2020). PubMed: 32355159
- Mishra S et al. Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications. Cell 174:803-817.e16 (2018). PubMed: 30057114
- Scapulatempo-Neto C et al. Characterization of topoisomerase II a and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13:1891-1898 (2017). IHC-P ; Human . PubMed: 28454340
- Zhang AT et al. Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication. J Cell Sci 124:2058-69 (2011). PubMed: 21610089
- Shtutman M et al. Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing. Proc Natl Acad Sci U S A 107:7377-82 (2010). WB ; Human . PubMed: 20368428
- Sarfraz S et al. Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection. BMC Microbiol 8:133 (2008). IHC-P ; Human . PubMed: 18680610
- Villadsen R et al. Evidence for a stem cell hierarchy in the adult human breast. J Cell Biol 177:87-101 (2007). PubMed: 17420292
- Niklaus AL et al. Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women. Hum Reprod 22:1778-88 (2007). PubMed: 17371803
- Canela N et al. Identification of an hexapeptide that binds to a surface pocket in cyclin A and inhibits the catalytic activity of the complex cyclin-dependent kinase 2-cyclin A. J Biol Chem 281:35942-53 (2006). WB ; Human . PubMed: 17001081
- Duursma A & Agami R p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation. Mol Cell Biol 25:6937-47 (2005). WB . PubMed: 16055707