Recombinant Anti-MCM2 antibody [EPR3727] (ab133325)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3727] to MCM2
- Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MCM2 antibody [EPR3727]
See all MCM2 primary antibodies -
Description
Rabbit monoclonal [EPR3727] to MCM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat, and MCF-7 cell lysates; Human cervical carcinoma and tonsil tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3727 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/200.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/200. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
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Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
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All lanes : Anti-MCM2 antibody [EPR3727] (ab133325) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab133325 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling MCM2 with ab133325 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MCM2 with ab133325 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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MCM2 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) with ab133325 at 1/60 dilution.
Exposue time:10 second.
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Immunofluorescent analysis of HeLa cells labelling MCM2 with ab133325 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133325 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab133325 has been referenced in 3 publications.
- Liang Y et al. Protein kinase D1 phosphorylation of KAT7 enhances its protein stability and promotes replication licensing and cell proliferation. Cell Death Discov 6:89 (2020). PubMed: 33014433
- Chan YT et al. Concordant and discordant regulation of target genes by miR-31 and its isoforms. PLoS One 8:e58169 (2013). WB ; Human . PubMed: 23472152
- Ishikawa J et al. Suppressive effects of liquid crystal compounds on the growth of U937 human leukemic monocyte lymphoma cells. Cancer Cell Int 12:3 (2012). WB . PubMed: 22300067