RecombinantRabMAb

Recombinant Anti-MCM2 antibody [EPR3727] (ab133325)

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Western blot - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Flow Cytometry - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunoprecipitation - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
  • Anti-MCM2 antibody [EPR3727] (ab133325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3727] to MCM2
  • Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-MCM2 antibody [EPR3727]
    See all MCM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3727] to MCM2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, and MCF-7 cell lysates; Human cervical carcinoma and tonsil tissues; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3727

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/200.
Notes
Flow Cyt
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/200.

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.

  • Post-translational
    modifications

    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Images

    • Western blot - Anti-MCM2 antibody [EPR3727] (ab133325)
      Western blot - Anti-MCM2 antibody [EPR3727] (ab133325)
      All lanes : Anti-MCM2 antibody [EPR3727] (ab133325) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF-7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa

    • Flow Cytometry - Anti-MCM2 antibody [EPR3727] (ab133325)
      Flow Cytometry - Anti-MCM2 antibody [EPR3727] (ab133325)

      Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab133325 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling MCM2 with ab133325 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MCM2 with ab133325 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunoprecipitation - Anti-MCM2 antibody [EPR3727] (ab133325)

      MCM2 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) with ab133325 at 1/60 dilution.

       

      Exposue time:10 second.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunofluorescent analysis of HeLa cells labelling MCM2 with ab133325 at 1/100 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133325 showing -ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133325 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133325 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab133325 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab133325 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

      Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133325 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-MCM2 antibody [EPR3727] (ab133325)
      Anti-MCM2 antibody [EPR3727] (ab133325)

    References (2)

    Publishing research using ab133325? Please let us know so that we can cite the reference in this datasheet.

    ab133325 has been referenced in 2 publications.

    • Chan YT  et al. Concordant and discordant regulation of target genes by miR-31 and its isoforms. PLoS One 8:e58169 (2013). WB ; Human . PubMed: 23472152
    • Ishikawa J  et al. Suppressive effects of liquid crystal compounds on the growth of U937 human leukemic monocyte lymphoma cells. Cancer Cell Int 12:3 (2012). WB . PubMed: 22300067

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