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    mcm2-antibody-epr4120-ab108935.pdf

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RecombinantRabMAb

Recombinant Anti-MCM2 antibody [EPR4120] (ab108935)

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Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
  • OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (ab108935)
  • Anti-MCM2 antibody [EPR4120] (ab108935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4120] to MCM2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-MCM2 antibody [EPR4120]
    See all MCM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4120] to MCM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF-7, Ramos, SW480, Molt-4, Jurkat and HeLa whole cell lysate (ab150035) IHC-P: Human squamous cell cervical carcinoma tissue and Human tonsil tissue IF: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 5.80 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4120
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-MCM2 antibody [EPR4120] (ab223402)
    • Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120] (ab223403)
    • Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.

For unpurified, use 1/1000 - 1/5000.

IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified, use 1/100.

ICC/IF
1/1000.

For unpurified, use 1/250.

Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.

For unpurified, use 1/1000 - 1/5000.

IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified, use 1/100.

ICC/IF
1/1000.

For unpurified, use 1/250.

Flow Cyt (Intra)
Use at an assay dependent concentration.

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications

    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P49736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4171 Human
    • Entrez Gene: 17216 Mouse
    • Entrez Gene: 312538 Rat
    • Omim: 116945 Human
    • SwissProt: P49736 Human
    • SwissProt: P97310 Mouse
    • Unigene: 477481 Human
    • Unigene: 16711 Mouse
    • Alternative names

      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Images

    • Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution (unpurified)

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : Molt-4 cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 102 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling MCM2 with purified ab108935 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395, at a 1/250 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

      Unpurified ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    • Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Mouse kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 125 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 125 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] (ab108935)
      Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] (ab108935)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab108935 at 1/160 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

       

       

    • Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
      All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/5000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Mouse kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L)

      Predicted band size: 102 kDa
      Observed band size: 125 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395, at a 1/250 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

      Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    • OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (ab108935)
      OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (ab108935)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-MCM2 antibody [EPR4120] (ab108935)
      Anti-MCM2 antibody [EPR4120] (ab108935)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab108935? Please let us know so that we can cite the reference in this datasheet.

    ab108935 has been referenced in 10 publications.

    • Lei Y  et al. Identification of MCM family as potential therapeutic and prognostic targets for hepatocellular carcinoma based on bioinformatics and experiments. Life Sci 272:119227 (2021). PubMed: 33607151
    • Zewdu R  et al. An NKX2-1/ERK/WNT feedback loop modulates gastric identity and response to targeted therapy in lung adenocarcinoma. Elife 10:N/A (2021). PubMed: 33821796
    • Zhang T  et al. APC mutations in human colon lead to decreased neuroendocrine maturation of ALDH+ stem cells that alters GLP-2 and SST feedback signaling: Clue to a link between WNT and retinoic acid signalling in colon cancer development. PLoS One 15:e0239601 (2020). PubMed: 33112876
    • Xu S  et al. Upregulation of microRNA-194-5p inhibits hypopharyngeal carcinoma cell proliferation, migration and invasion by targeting SMURF1 via the mTOR signaling pathway. Int J Oncol 54:1245-1255 (2019). PubMed: 30720112
    • Fourie C  et al. The paracrine effects of fibroblasts on Doxorubicin-treated breast cancer cells. Exp Cell Res 381:280-287 (2019). PubMed: 31121155
    • Abram TJ  et al. Risk Stratification of Oral Potentially Malignant Disorders in Fanconi Anemia Patients Using Autofluorescence Imaging and Cytology-On-A Chip Assay. Transl Oncol 11:477-486 (2018). PubMed: 29481998
    • Nakamura A  et al. CDK8/19 inhibition induces premature G1/S transition and ATR-dependent cell death in prostate cancer cells. Oncotarget 9:13474-13487 (2018). PubMed: 29568371
    • Jin Y & Dai Z USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells. Biomed Pharmacother 78:264-71 (2016). WB ; Human . PubMed: 26898451
    • Bai G  et al. Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation. PLoS Genet 12:e1005787 (2016). WB ; Mouse . PubMed: 26765334
    • Erbayraktar Z  et al. Cell division cycle 7-kinase inhibitor PHA-767491 hydrochloride suppresses glioblastoma growth and invasiveness. Cancer Cell Int 16:88 (2016). WB ; Human . PubMed: 27891063

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