Product nameAnti-MCM2 antibody - N-terminal
See all MCM2 primary antibodies
DescriptionRabbit polyclonal to MCM2 - N-terminal
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human MCM2 (N terminal). The exact sequence is proprietary.
Database link: P49736
- WB: HCT 116 whole cell lysate. IP: HCT 116 whole cell extract. ICC/IF: HCT 116 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 102 kDa.|
|IP||1/100 - 1/500.|
|ICC/IF||1/100 - 1/1000.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Information by UniProt
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
Anti-MCM2 antibody - N-terminal (ab227278) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 102 kDa
7.5% SDS-PAGE gel.
MCM2 was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) whole cell lysate with 4 μg ab227278. Western blot was performed from the immunoprecipitate using ab227278 at 1/500 dilution.
Lane 1: HCT 116 whole cell extract 40 μg (Input).
Lane 2: Preimmune Rabbit IgG instead of ab227278 in HCT 116 whole cell extract.
Lane 3: ab227278 IP in HCT 116 whole cell extract.
4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for MCM2 (green) using ab227278 at 1/500 dilution in ICC/IF. Counterstain: Phalloidin cytoskeletal marker at 1/50 dilution.
ab227278 has not yet been referenced specifically in any publications.