Recombinant Anti-MCM2 antibody [SP85] (ab95361)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP85] to MCM2
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MCM2 antibody [SP85]
See all MCM2 primary antibodies -
Description
Rabbit monoclonal [SP85] to MCM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide within Human MCM2 aa 100-200. The exact sequence is proprietary.
Database link: P49736 -
Positive control
- WB: HeLa cell lysate. IHC-P: Human rectal carcinoma, human pancreas adenocarcinoma and human prostate tissue. ICC/IF: HeLa, NIH/3T3 and C6 cells. Flow Cyt (Intra): HeLa, NIH/3T3 and C6 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP85 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IHC-P |
1/100. Antigen retrieval is recommended by the following method: boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.
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Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/50.
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Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
1/100. Antigen retrieval is recommended by the following method: boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. |
Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling MCM2 with purified ab95361 at 1/50 (2.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling MCM2 with purified ab95361 at 1/200 dilution (0.615 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
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Anti-MCM2 antibody [SP85] (ab95361) at 1/100 dilution + lysate prepared from HeLa cells
Observed band size: 130 kDa why is the actual band size different from the predicted? -
ab95361, at 1/100 dilution, staining MCM2 in formalin-fixed, paraffin-embedded Human rectal carcinoma tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling MCM2 with purified ab95361 at 1/50 (2.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling MCM2 with purified ab95361 at 1/50 (2.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of C6 (rat glial tumor glial cell) labeling MCM2 with purified ab95361 at 1/200 dilution (0.615 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
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Flow cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) labeling MCM2 with purified ab95361 at 1/200 dilution (0.615 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
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Flow cytometric analysis using rabbit anti-MCM2 (SP85) antibody ab95361(1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue).
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ab95361,at 1/100 dilution,staining MCM2 in formalin-fixed,paraffin-embedded Human pancreas adenocarcinoma tissue by Immunohistochemistry.
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ab95361,at 1/100 dilution,staining MCM2 in formalin-fixed,paraffin-embedded Human prostate tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab95361 has been referenced in 2 publications.
- Yap K et al. Hybridization-proximity labeling reveals spatially ordered interactions of nuclear RNA compartments. Mol Cell 82:463-478.e11 (2022). PubMed: 34741808
- Li KY et al. Morphological and molecular alterations of reactive astrocytes without proliferation in cerebral cortex of an APP/PS1 transgenic mouse model and Alzheimer's patients. Glia N/A:N/A (2020). PubMed: 32469469