The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min).
Incubate for 30 minutes at 4°C.Optimal dilution and procedure should be determined by the end user.
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Belongs to the MCM family. Contains 1 MCM domain.
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.