Recombinant Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
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Overview
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Product name
Anti-MCM2 (phospho S108) antibody [EPR4121]
See all MCM2 primary antibodies -
Description
Rabbit monoclonal [EPR4121] to MCM2 (phospho S108) -
Host species
Rabbit -
Specificity
ab109271 only detects MCM2 phosphorylated at serine 108. -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Phospho specific peptide corresponding to residues surrounding Serine 108 of Human MCM2.
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Positive control
- HeLa whole cell lysate (ab150035); HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4121 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa). | |
| IP | 1/10 - 1/100. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
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![Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-122006-anti-mcm2-phospho-s108-antibody-epr4121-western-blot.jpg)
Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)All lanes : Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-122005-anti-mcm2-phospho-s108-antibody-epr4121-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)Immunofluorescent staining of HeLa cells using ab109271 at a dilution of 1/100. -
![Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-266550-anti-mcm2-phospho-s108-antibody-epr4121-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)MCM2 was immunoprecipitated from Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) with ab109271 at 1/80 dilution.
Exposue time:10 second.
Protocols
Datasheets and documents
References
ab109271 has not yet been referenced specifically in any publications.
