Recombinant Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
![Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-122006-anti-mcm2-phospho-s108-antibody-epr4121-western-blot.jpg "Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4121] to MCM2 (phospho S108)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-MCM2 (phospho S108) antibody [EPR4121]
See all MCM2 primary antibodies -
Description
Rabbit monoclonal [EPR4121] to MCM2 (phospho S108) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa whole cell lysate (ab150035); HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4121 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).
|
|
| IP |
1/10 - 1/100.
|
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa). |
|
IP
1/10 - 1/100. |
|
ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
-
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
-
Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
-
Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)All lanes : Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-122005-anti-mcm2-phospho-s108-antibody-epr4121-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)Immunofluorescent staining of HeLa cells using ab109271 at a dilution of 1/100. -
![Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-266550-anti-mcm2-phospho-s108-antibody-epr4121-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)MCM2 was immunoprecipitated from Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) with ab109271 at 1/80 dilution.
Exposue time:10 second.
-
![Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-4-benefits-of-recombinant-antibodies.png)
Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
Protocols
Datasheets and documents
-
Datasheet download
References (3)
ab109271 has been referenced in 3 publications.
- Zhang J et al. DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. Nat Commun 11:3951 (2020). PubMed: 32769987
- Mela M et al. The Alpha-1 Antitrypsin Polymer Load Correlates With Hepatocyte Senescence, Fibrosis Stage and Liver-Related Mortality. Chronic Obstr Pulm Dis 7:151-162 (2020). PubMed: 32726073
- Huang J et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
