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RecombinantRabMAb

Recombinant Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

  • Datasheet
  • SDS
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Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
  • Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
  • Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4121] to MCM2 (phospho S108)
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-MCM2 (phospho S108) antibody [EPR4121]
    See all MCM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4121] to MCM2 (phospho S108)
  • Host species

    Rabbit
  • Specificity

    ab109271 only detects MCM2 phosphorylated at serine 108.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Serine 108 of Human MCM2.

  • Positive control

    • HeLa whole cell lysate (ab150035); HeLa cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4121
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Alternative Versions

    • Anti-MCM2 (phospho S108) antibody [EPR4121] - BSA and Azide free (ab247816)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IP
Mouse
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).
IP
1/10 - 1/100.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).
IP
1/10 - 1/100.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • Sequence similarities

      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications

      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P49736 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4171 Human
      • Entrez Gene: 17216 Mouse
      • Entrez Gene: 312538 Rat
      • Omim: 116945 Human
      • SwissProt: P49736 Human
      • SwissProt: P97310 Mouse
      • Unigene: 477481 Human
      • Unigene: 16711 Mouse
      • Alternative names

        • BM28 antibody
        • CCNL 1 antibody
        • CCNL1 antibody
        • CDC like 1 antibody
        • cdc19 antibody
        • CDCL 1 antibody
        • CDCL1 antibody
        • Cell devision cycle like 1 antibody
        • Cyclin like 1 antibody
        • D3S3194 antibody
        • DNA replication licensing factor MCM2 antibody
        • KIAA0030 antibody
        • MCM 2 antibody
        • MCM2 antibody
        • MCM2 minichromosome maintenance deficient 2 mitotin antibody
        • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
        • MCM2 minichromosome maintenance deficient 2, mitotin antibody
        • MCM2_HUMAN antibody
        • MGC10606 antibody
        • Minichromosome maintenance complex component 2 antibody
        • Minichromosome maintenance deficient 2 (mitotin) antibody
        • Minichromosome maintenance deficient 2 mitotin antibody
        • Minichromosome maintenance protein 2 antibody
        • Minichromosome maintenance protein 2 homolog antibody
        • Mitotin antibody
        • Nuclear protein BM28 antibody
        • OTTHUMP00000216047 antibody
        • OTTHUMP00000216050 antibody
        see all

      Images

      • Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
        Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
        All lanes : Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilution

        Lane 1 : HeLa cell lysate, untreated
        Lane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 102 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
        Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
        Immunofluorescent staining of HeLa cells using ab109271 at a dilution of 1/100.
      • Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
        Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

        MCM2 was immunoprecipitated from Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) with ab109271 at 1/80 dilution.

        Exposue time:10 second.

      • Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
        Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab109271? Please let us know so that we can cite the reference in this datasheet.

      ab109271 has been referenced in 1 publication.

      • Huang J  et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464

      Customer reviews and Q&As

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