Recombinant Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
![Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-122006-anti-mcm2-phospho-s108-antibody-epr4121-western-blot.jpg "Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4121] to MCM2 (phospho S108)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MCM2 (phospho S108) antibody [EPR4121]
See all MCM2 primary antibodies -
Description
Rabbit monoclonal [EPR4121] to MCM2 (phospho S108) -
Host species
Rabbit -
Specificity
ab109271 only detects MCM2 phosphorylated at serine 108. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP MouseWB Human
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Immunogen
Phospho specific peptide corresponding to residues surrounding Serine 108 of Human MCM2.
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Positive control
- HeLa whole cell lysate (ab150035); HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4121 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IP |
Mouse
|
| WB |
Human
|
| All applications |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).
|
|
| IP |
1/10 - 1/100.
|
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa). |
|
IP
1/10 - 1/100. |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
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Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)All lanes : Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-122005-anti-mcm2-phospho-s108-antibody-epr4121-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)Immunofluorescent staining of HeLa cells using ab109271 at a dilution of 1/100. -
![Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-266550-anti-mcm2-phospho-s108-antibody-epr4121-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)MCM2 was immunoprecipitated from Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) with ab109271 at 1/80 dilution.
Exposue time:10 second.
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![Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)](https://www.abcam.com/ps/products/109/ab109271/Images/ab109271-4-benefits-of-recombinant-antibodies.png)
Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109271 has been referenced in 1 publication.
- Huang J et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464