Product nameAnti-MCM2 (phospho S40) antibody [EPR4170(2)]
See all MCM2 primary antibodies
DescriptionRabbit monoclonal [EPR4170(2)] to MCM2 (phospho S40)
SpecificityThis antibody only detects MCM2 phosphorylated at serine 40.
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding serine 40 of human MCM2 (P49736).
- HeLa whole cell lysate (ab150035).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 102 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Information by UniProt
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
All lanes : Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (ab133243) at 1/1000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate alkaline phosphatase treated
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling MCM2 (phospho S40) with ab133243 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Nakamura A et al. CDK8/19 inhibition induces premature G1/S transition and ATR-dependent cell death in prostate cancer cells. Oncotarget 9:13474-13487 (2018). Read more (PubMed: 29568371) »
- Rodriguez-Acebes S et al. Uncoupling fork speed and origin activity to identify the primary cause of replicative stress phenotypes. J Biol Chem 293:12855-12861 (2018). Read more (PubMed: 29959228) »