Key features and details
- Rabbit polyclonal to MCM2 (phospho S40 + S41)
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCM2 (phospho S40 + S41) antibody
See all MCM2 primary antibodies
DescriptionRabbit polyclonal to MCM2 (phospho S40 + S41)
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
A synthetic di-phosphorylated peptide, corresponding to a sequence from human MCM2 surrounding Serines 40 and 41. (NP_004517.2)
- Whole cell lysate from asynchronous 293T cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 100 kDa).|
|Flow Cyt||Use 0.1µg for 106 cells.
0.1 μg per 1 X 106 cells in a 150 µl volume.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Information by UniProt
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
All lanes : Anti-MCM2 (phospho S40 + S41) antibody (ab70371) at 0.1 µg/ml
Lane 1 : Whole cell lysate from asynchronous 293T cells
Lane 2 : Whole cell lysate from asynchronous 293T cells treated with a phosphatase
Lysates/proteins at 50 µg per lane.
Predicted band size: 100 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
The bottom panel shows total MCM2 staining; the blot was stripped and then blotted with rabbit anti-total MCM2 antibody at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds for upper and lower panel.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right) tissues labelling MCM2 (phospho S40 + S41) with ab70371 at 1/200 (1µg/ml). Detection: DAB.
ab70371 at 0.1µg/ml staining MCM2 (phospho S40 + S41) by Flow Cytometery. 1x106 cells were fixed in 1.5% PFA and permeabilized in 90% MeOH. DNA content was simultaneously analyzed via PI stain.
ab70371 has been referenced in 2 publications.
- McLaughlin RP et al. A kinase inhibitor screen identifies a dual cdc7/CDK9 inhibitor to sensitise triple-negative breast cancer to EGFR-targeted therapy. Breast Cancer Res 21:77 (2019). PubMed: 31262335
- Erbayraktar Z et al. Cell division cycle 7-kinase inhibitor PHA-767491 hydrochloride suppresses glioblastoma growth and invasiveness. Cancer Cell Int 16:88 (2016). WB ; Human . PubMed: 27891063