Product nameAnti-MCM3 antibody
See all MCM3 primary antibodies
DescriptionRabbit polyclonal to MCM3
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human MCM3. Center region.
Database link: P25205
- WB: HepG2 whole cell lysate. IHC-P: Mouse colon tissue. ICC/IF: HeLa cells. IP: HepG2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|IP||1/100 - 1/500.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
Anti-MCM3 antibody (ab272877) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Paraffin-embedded mouse colon tissue stained for MCM3 using ab272877 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MCM3 using ab272877 at 1/500 dilution in ICC/IF.
Red: alpha Tubulin, a cytoskeleton marker.
Blue: Hoechst 33342 staining.
Samples: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
A. 40 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM3 protein by 4 μg of ab272877
7.5 % SDS-PAGE
The immunoprecipitated MCM3 protein was detected by ab272877 diluted at 1:1000, followed by anti-rabbit IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272877 has not yet been referenced specifically in any publications.