Overview

  • Product name
  • Description
    Goat polyclonal to MCM3
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Pig, Caenorhabditis elegans, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 10 and 11.

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 90 kDa).

Target

  • Function
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cervical cancer proto oncogene 5 antibody
    • DNA polymerase alpha holoenzyme associated P1 antibody
    • DNA polymerase alpha holoenzyme associated protein P1 antibody
    • DNA polymerase alpha holoenzyme-associated protein P1 antibody
    • DNA replication factor MCM3 antibody
    • DNA replication licensing factor mcm3 antibody
    • HCC 5 antibody
    • HCC5 antibody
    • hRlf beta subunit antibody
    • Human cervical cancer proto oncogene 5 antibody
    • MCM 3 antibody
    • mcm3 antibody
    • MCM3 minichromosome maintenance deficient 3 antibody
    • MCM3_HUMAN antibody
    • MGC1157 antibody
    • Minichromosome maintenance complex component 3 antibody
    • Minichromosome maintenance deficient 3 antibody
    • Minichromosome maintenance protein 3 antibody
    • P1 h antibody
    • P1 MCM3 antibody
    • P1 Protein antibody
    • P1-MCM3 antibody
    • P1.h antibody
    • p102 antibody
    • P102 protein antibody
    • Replication licensing factor beta subunit antibody
    • RLF beta subunit antibody
    • RLF subunit beta antibody
    • RLFB antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling MCM3 with ab3725 at 1/1000 (1µg/ml). Detection: DAB.
  • Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 3 (ab3725) diluted 1 / 4000.

    Detection: ECL.

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 3 (ab3725) diluted 1 / 4000.

    Detection: ECL.

References

ab3725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. There is a very low probability that ab3724 would react with Drosophila MCM3 and very low to zero probability that ab3725 or ab4460 would react too. I hope this information will be useful. Should you require any other assistance, please do not hesitate to contact me.

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Answer

Unfortunately, we are not specialists in this area and we do not want to recommend a product that is unsuitable for your needs. We suggest that you refer to the latest literature in this field. Doing a quick search, a thought may be Ep-CAM (the following reference stated that "Normal endocervical glandular epithelium (Both columnar and reserve cells) appeared strongly positive for EP-CAM") - American Journal of Pathology, Vol 148, 865-875, Copyright © 1996 by American Society for Investigative Pathology.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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