Key features and details
- Rabbit polyclonal to MCM3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCM3 antibody
See all MCM3 primary antibodies
DescriptionRabbit polyclonal to MCM3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment containing a sequence corresponding to a region within amino acids 42-272 of Human MCM3 (NP_002379).
- HepG2 whole cell lysate; HeLa cells; Cal27 xenograft; 293T, A431, H1299, Molt-4 and Raji cell lines
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 91 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
Anti-MCM3 antibody (ab97282) at 1/10000 dilution + HepG2 whole lysate at 30 µg
Predicted band size: 91 kDa
7.5% SDS PAGE
Immunofluorescence analysis of MCM3 in paraformaldehyde-fixed HeLa cells, using ab97282 at 1/500 dilution. Lower image is merged with DNA probe.
Immunohistochemical analysis of MCM3 in paraffin-embedded Cal27 xenograft, using ab97282 at 1/500 dilution.
ab97282 has not yet been referenced specifically in any publications.