Overview

  • Product name

    Anti-MCM3 antibody - C-terminal
    See all MCM3 primary antibodies
  • Description

    Rabbit polyclonal to MCM3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MCM3 (C terminal). The exact sequence is proprietary.
    Database link: P25205

  • Positive control

    • WB: HEK-293T whole cell lysate. IHC: NCIC87 xenograft. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 91 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cervical cancer proto oncogene 5 antibody
    • DNA polymerase alpha holoenzyme associated P1 antibody
    • DNA polymerase alpha holoenzyme associated protein P1 antibody
    • DNA polymerase alpha holoenzyme-associated protein P1 antibody
    • DNA replication factor MCM3 antibody
    • DNA replication licensing factor mcm3 antibody
    • HCC 5 antibody
    • HCC5 antibody
    • hRlf beta subunit antibody
    • Human cervical cancer proto oncogene 5 antibody
    • MCM 3 antibody
    • mcm3 antibody
    • MCM3 minichromosome maintenance deficient 3 antibody
    • MCM3_HUMAN antibody
    • MGC1157 antibody
    • Minichromosome maintenance complex component 3 antibody
    • Minichromosome maintenance deficient 3 antibody
    • Minichromosome maintenance protein 3 antibody
    • P1 h antibody
    • P1 MCM3 antibody
    • P1 Protein antibody
    • P1-MCM3 antibody
    • P1.h antibody
    • p102 antibody
    • P102 protein antibody
    • Replication licensing factor beta subunit antibody
    • RLF beta subunit antibody
    • RLF subunit beta antibody
    • RLFB antibody
    see all

Images

  • Anti-MCM3 antibody - C-terminal (ab228778) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 91 kDa



    7.5% SDS-PAGE

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MCM3 (green) using ab228778 at 1/500 in ICC/IF.

    Nuclear counterstain: Hoechst 33343 (blue).

  • Paraffin-embedded NCIN87 xenograft stained for MCM3 using ab228778 at 1/100 dilution in immunohistochemical analysis.

References

ab228778 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228778.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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