Overview

  • Product name

    Anti-MCM3 antibody [EPR7080]
    See all MCM3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7080] to MCM3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human MCM3 (P25205).

  • Positive control

    • L6, C6, RAW 264.7, PC-12, NIH/3T3, HeLa, K562, HepG2, U937 and 293T cell lysates. Human colon and tonsil tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7080
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
    • Sequence similarities

      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cervical cancer proto oncogene 5 antibody
      • DNA polymerase alpha holoenzyme associated P1 antibody
      • DNA polymerase alpha holoenzyme associated protein P1 antibody
      • DNA polymerase alpha holoenzyme-associated protein P1 antibody
      • DNA replication factor MCM3 antibody
      • DNA replication licensing factor mcm3 antibody
      • HCC 5 antibody
      • HCC5 antibody
      • hRlf beta subunit antibody
      • Human cervical cancer proto oncogene 5 antibody
      • MCM 3 antibody
      • mcm3 antibody
      • MCM3 minichromosome maintenance deficient 3 antibody
      • MCM3_HUMAN antibody
      • MGC1157 antibody
      • Minichromosome maintenance complex component 3 antibody
      • Minichromosome maintenance deficient 3 antibody
      • Minichromosome maintenance protein 3 antibody
      • P1 h antibody
      • P1 MCM3 antibody
      • P1 Protein antibody
      • P1-MCM3 antibody
      • P1.h antibody
      • p102 antibody
      • P102 protein antibody
      • Replication licensing factor beta subunit antibody
      • RLF beta subunit antibody
      • RLF subunit beta antibody
      • RLFB antibody
      see all

    Images

    • All lanes : Anti-MCM3 antibody [EPR7080] (ab128923) at 1/1000 dilution

      Lane 1 : L6 cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : RAW 264.7 cell lysate
      Lane 4 : PC-12 cell lysate
      Lane 5 : NIH/3T3 cell lysate
      Lane 6 : HeLa cell lysate
      Lane 7 : K562 cell lysate
      Lane 8 : HepG2 cell lysate
      Lane 9 : U937 cell lysate
      Lane 10 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 91 kDa

    • ab128923 showing positive staining in Normal colon tissue.

    • ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human tonsil tissue by immunohistochemistry.
    • ab128923 showing positive staining in Breast carcinoma tissue.

    • ab128923 showing negative staining in Normal brain tissue.

    • ab128923 showing positive staining in Lung carcinoma tissue.

    • Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling MCM3 with unpurified ab128923 at 1/900 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • ab128923 showing positive staining in Cervical carcinoma tissue.

    References

    This product has been referenced in:

    • Zhang K  et al. Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis. Cell Death Dis 9:1035 (2018). Read more (PubMed: 30305611) »
    • Ye Y  et al. Up-regulation of REG3A in colorectal cancer cells confers proliferation and correlates with colorectal cancer risk. Oncotarget 7:3921-33 (2016). WB . Read more (PubMed: 26646797) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab128923.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up