RecombinantRabMAb

Recombinant Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

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Western blot - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Flow Cytometry - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
  • Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7080] to MCM3 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MCM3 antibody [EPR7080] - BSA and Azide free
    See all MCM3 primary antibodies

  • Description

    Rabbit monoclonal [EPR7080] to MCM3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MCM3. The exact sequence is proprietary.
    Database link: P25205

  • General notes

    Ab248222 is the carrier-free version of ab128923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248222 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7080

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab248222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

    • Sequence similarities

      Belongs to the MCM family.
      Contains 1 MCM domain.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cervical cancer proto oncogene 5 antibody
        • DNA polymerase alpha holoenzyme associated P1 antibody
        • DNA polymerase alpha holoenzyme associated protein P1 antibody
        • DNA polymerase alpha holoenzyme-associated protein P1 antibody
        • DNA replication factor MCM3 antibody
        • DNA replication licensing factor mcm3 antibody
        • HCC 5 antibody
        • HCC5 antibody
        • hRlf beta subunit antibody
        • Human cervical cancer proto oncogene 5 antibody
        • MCM 3 antibody
        • mcm3 antibody
        • MCM3 minichromosome maintenance deficient 3 antibody
        • MCM3_HUMAN antibody
        • MGC1157 antibody
        • Minichromosome maintenance complex component 3 antibody
        • Minichromosome maintenance deficient 3 antibody
        • Minichromosome maintenance protein 3 antibody
        • P1 h antibody
        • P1 MCM3 antibody
        • P1 Protein antibody
        • P1-MCM3 antibody
        • P1.h antibody
        • p102 antibody
        • P102 protein antibody
        • Replication licensing factor beta subunit antibody
        • RLF beta subunit antibody
        • RLF subunit beta antibody
        • RLFB antibody
        see all

      Images

      • Western blot - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Western blot - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        All lanes : Anti-MCM3 antibody [EPR7080] (ab128923) at 1/1000 dilution

        Lane 1 : L6 cell lysate
        Lane 2 : C6 cell lysate
        Lane 3 : RAW 264.7 cell lysate
        Lane 4 : PC-12 cell lysate
        Lane 5 : NIH/3T3 cell lysate
        Lane 6 : HeLa cell lysate
        Lane 7 : K562 cell lysate
        Lane 8 : HepG2 cell lysate
        Lane 9 : U937 cell lysate
        Lane 10 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 91 kDa



        This data was developed using ab128923, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

        This data was developed using ab128923, the same antibody clone in a different buffer formulation.

        ab128923 showing positive staining in Normal colon tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

        This data was developed using ab128923, the same antibody clone in a different buffer formulation.

        ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

        This data was developed using ab128923, the same antibody clone in a different buffer formulation.

        ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human tonsil tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing positive staining in Breast carcinoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing negative staining in Normal brain tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing positive staining in Lung carcinoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Flow Cytometry - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Flow Cytometry - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        This data was developed using ab128923, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling MCM3 with unpurified ab128923 at 1/900 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        This data was developed using ab128923, the same antibody clone in a different buffer formulation.ab128923 showing positive staining in Cervical carcinoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
        Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248222? Please let us know so that we can cite the reference in this datasheet.

      ab248222 has not yet been referenced specifically in any publications.

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