Recombinant Anti-MCM3 antibody [EPR7080] - BSA and Azide free (ab248222)
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- References
- Protocols
Overview
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Product name
Anti-MCM3 antibody [EPR7080] - BSA and Azide free
See all MCM3 primary antibodies -
Description
Rabbit monoclonal [EPR7080] to MCM3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MCM3. The exact sequence is proprietary.
Database link: P25205 -
General notes
Ab248222 is the carrier-free version of ab128923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248222 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7080 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab248222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa). | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4172 Human
- Entrez Gene: 17215 Mouse
- Entrez Gene: 316273 Rat
- Omim: 602693 Human
- SwissProt: P25205 Human
- SwissProt: P25206 Mouse
- Unigene: 179565 Human
- Unigene: 4502 Mouse
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Alternative names
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
see all
Datasheets and documents
References
ab248222 has not yet been referenced specifically in any publications.
