Overview

  • Product name

    Anti-MCM3 antibody [EPR7081]
    See all MCM3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7081] to MCM3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MCM3 aa 750-850. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, and 293T cell lysates; 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7081
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
    • Sequence similarities

      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cervical cancer proto oncogene 5 antibody
      • DNA polymerase alpha holoenzyme associated P1 antibody
      • DNA polymerase alpha holoenzyme associated protein P1 antibody
      • DNA polymerase alpha holoenzyme-associated protein P1 antibody
      • DNA replication factor MCM3 antibody
      • DNA replication licensing factor mcm3 antibody
      • HCC 5 antibody
      • HCC5 antibody
      • hRlf beta subunit antibody
      • Human cervical cancer proto oncogene 5 antibody
      • MCM 3 antibody
      • mcm3 antibody
      • MCM3 minichromosome maintenance deficient 3 antibody
      • MCM3_HUMAN antibody
      • MGC1157 antibody
      • Minichromosome maintenance complex component 3 antibody
      • Minichromosome maintenance deficient 3 antibody
      • Minichromosome maintenance protein 3 antibody
      • P1 h antibody
      • P1 MCM3 antibody
      • P1 Protein antibody
      • P1-MCM3 antibody
      • P1.h antibody
      • p102 antibody
      • P102 protein antibody
      • Replication licensing factor beta subunit antibody
      • RLF beta subunit antibody
      • RLF subunit beta antibody
      • RLFB antibody
      see all

    Images

    • All lanes : Anti-MCM3 antibody [EPR7081] (ab126723) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 91 kDa

    • ab126723, at 1/10 dilution staining MCM3 in permeabilized 293T cells by Flow Cytometry (red). A rabbit IgG negative control is shown in green.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126723 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126723.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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