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    mcm4-antibody-ab4459.pdf

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Anti-MCM4 antibody (ab4459)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (22)

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Western blot - Anti-MCM4 antibody (ab4459)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
  • Western blot - Anti-MCM4 antibody (ab4459)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)

Key features and details

  • Rabbit polyclonal to MCM4
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MCM4 antibody
    See all MCM4 primary antibodies
  • Description

    Rabbit polyclonal to MCM4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (Human) - 32 amino acids which represented a portion of human MCM 4 encoded within exon 14.

  • Positive control

    • IHC-P: FFPE human testis tissue sections.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Rabbit
Horse
Guinea pig
Cow
Chimpanzee
Drosophila melanogaster
Rhesus monkey
Gorilla
Opossum
Orangutan
Elephant
Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use at an assay dependent concentration.
WB (1)
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 96.6 kDa).
Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use at an assay dependent concentration.
WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 96.6 kDa).

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P33991 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 35679 Drosophila melanogaster
    • Entrez Gene: 4173 Human
    • Omim: 602638 Human
    • SwissProt: Q26454 Drosophila melanogaster
    • SwissProt: P33991 Human
    • Unigene: 21562 Drosophila melanogaster
    • Unigene: 460184 Human
    • Alternative names

      • 19G antibody
      • AI325074 antibody
      • AU045576 antibody
      • CDC 21 antibody
      • CDC 54 antibody
      • CDC21 antibody
      • CDC21 homolog antibody
      • CDC21, S. pombe, homolog of antibody
      • CDC54 antibody
      • Cell division cycle 21, S. pombe, homolog of antibody
      • DNA replication licensing factor MCM 4 antibody
      • DNA replication licensing factor MCM4 antibody
      • hCdc 21 antibody
      • hCdc21 antibody
      • Homolog of S. pombe cell devision cycle 21 antibody
      • KIAA4003 antibody
      • mcdc21 antibody
      • MCM 4 antibody
      • MCM 4 minichromosome maintenance deficient 4 antibody
      • mcm4 antibody
      • MCM4 minichromosome maintenance deficient 4 antibody
      • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
      • MCM4_HUMAN antibody
      • Mcmd4 antibody
      • MGC33310 antibody
      • Minichromosome maintenance 4 antibody
      • Minichromosome maintenance complex component 4 antibody
      • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
      • Minichromosome maintenance deficient 4 antibody
      • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
      • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
      • mKIAA4003 antibody
      • NKCD antibody
      • NKGCD antibody
      • P1 CDC21 antibody
      • P1-CDC21 antibody
      • P1CDC21 antibody
      see all

    Images

    • Western blot - Anti-MCM4 antibody (ab4459)
      Western blot - Anti-MCM4 antibody (ab4459)
      HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour. HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM4 with ab4459 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)

      IHC image of MCM4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-MCM4 antibody (ab4459)
      Western blot - Anti-MCM4 antibody (ab4459)
      Anti-MCM4 antibody (ab4459) at 1/2000 dilution + HeLa nuclear cell lysate. at 20 µg

      Secondary
      Goat anti rabbit HRP. at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 96.6 kDa



      This image is an edited version of an image received courtesy of an Abreview submitted by Jason DeHart on 22 August 2005. We do not have any further information relating to this image.
    • Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)
      ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (22)

    Publishing research using ab4459? Please let us know so that we can cite the reference in this datasheet.

    ab4459 has been referenced in 22 publications.

    • Prendergast L  et al. Histone chaperone FACT is essential to overcome replication stress in mammalian cells. Oncogene 39:5124-5137 (2020). PubMed: 32533099
    • Prikrylova T  et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). PubMed: 31363131
    • Starokadomskyy P  et al. NK cell defects in X-linked pigmentary reticulate disorder. JCI Insight 4:N/A (2019). PubMed: 31672938
    • Cortone G  et al. Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion. PLoS Genet 14:e1007622 (2018). PubMed: 30303954
    • Charrasse S  et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). PubMed: 28785014
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Hi,    I;m a graduate student from University of Southern California. Our lab works with Pombe/ fission yeast. I;m interested in using the mcm4 antibody (ab 4459) to see if it works with pombe. I would like to find out if there is any way that I can determine if the above mentioned antibody could be used in our system.Further if there if it will be possible to obtain a sample of the antibody to test before making a purchase    thanks

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2011

    Answer

    Thank you very much for your inquiry. I am sorry to confirm that I ran a blast search and could not find any homology information of the immunogen used to generate this antibody and Schizosaccharomyces pombe. The homology to Saccharomyces cerevisiae is only 50% and therefore I assume that this antibody is also not likely to cross-react with Schizosaccharomyces pombe. We expect cross-reactivity from 85% upwards. I am sorry I do not have a positive answer to your inquiry on this occasion and hope this information is nevertheless helpful.

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2011

    Western blot abreview for Anti-MCM4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 22 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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