Anti-MCM4 antibody (ab4459)
- Datasheet
- References (20)
- Protocols
Overview
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Product name
Anti-MCM4 antibody
See all MCM4 primary antibodies -
Description
Rabbit polyclonal to MCM4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human, Xenopus laevis
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
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Immunogen
Synthetic peptide (Human) - 32 amino acids which represented a portion of human MCM 4 encoded within exon 14.
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Positive control
- IHC-P: FFPE human testis tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab4459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 96.6 kDa). |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 35679 Drosophila melanogaster
- Entrez Gene: 4173 Human
- Entrez Gene: 17217 Mouse
- Omim: 602638 Human
- SwissProt: Q26454 Drosophila melanogaster
- SwissProt: P33991 Human
- SwissProt: P49717 Mouse
- Unigene: 21562 Drosophila melanogaster
see all -
Alternative names
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
Images
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Western blot - Anti-MCM4 antibody (ab4459)HeLa cell RIPA extract incubated with 0.2µ g/ml of ab4459 for 1 hour. HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM4 with ab4459 at 1/1000 (1µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)IHC image of MCM4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-MCM4 antibody (ab4459)Anti-MCM4 antibody (ab4459) at 1/2000 dilution + HeLa nuclear cell lysate. at 20 µg
Secondary
Goat anti rabbit HRP. at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 96.6 kDa
This image is an edited version of an image received courtesy of an Abreview submitted by Jason DeHart on 22 August 2005. We do not have any further information relating to this image. -
Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Prikrylova T et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). Read more (PubMed: 31363131) »
- Cortone G et al. Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion. PLoS Genet 14:e1007622 (2018). Read more (PubMed: 30303954) »
