Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5245] to MCM4
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-MCM4 antibody [EPR5245]
See all MCM4 primary antibodies
DescriptionRabbit monoclonal [EPR5245] to MCM4
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human MCM4 aa 800-900 (C terminal). The exact sequence is proprietary.
- HeLa, Molt-4, and A431 cell lysates and Molt-4 cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.80 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Predicted molecular weight: 97 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
- Information by UniProt
- 19G antibody
- AI325074 antibody
- AU045576 antibody
All lanes : Anti-MCM4 antibody [EPR5245] (ab124836) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 97 kDa
Flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red (ab124836) or a rabbit IgG in green(negative).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124836 has not yet been referenced specifically in any publications.