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Rabbit monoclonal [EPR5245] to MCM4
Flow Cyt more details Unsuitable for:
ICC/IF or IHC-P
Synthetic peptide within Human MCM4 aa 800-900 (C terminal). The exact sequence is proprietary.
HeLa, Molt-4, and A431 cell lysates and Molt-4 cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K
Preservative: 0.05% Sodium azide Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000 - 1/200000. Predicted molecular weight: 97 kDa.
1/10 - 1/100.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC/IF or IHC-P.
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Belongs to the MCM family.
Contains 1 MCM domain.
Information by UniProt
Western blot - Anti-MCM4 antibody [EPR5245] (ab124836)
All lanes : Anti-MCM4 antibody [EPR5245] (ab124836) at 1/50000 dilution Lane 1 : HeLa cell lysate Lane 2 : Molt-4 cell lysate Lane 3 : A431 cell lysate Lysates/proteins at 10 µg per lane. Predicted band size: 97 kDa
Flow Cytometry - Anti-MCM4 antibody [EPR5245] (ab124836)
Flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red (ab124836) or a rabbit IgG in green(negative).
Other - Anti-MCM4 antibody [EPR5245] (ab124836)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"