• Product name

    Anti-MCM4 antibody [EPR5245]
    See all MCM4 primary antibodies
  • Description

    Rabbit monoclonal [EPR5245] to MCM4
  • Host species

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MCM4 aa 800-900 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, Molt-4, and A431 cell lysates and Molt-4 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.80 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab124836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 97 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
    • Sequence similarities

      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • 19G antibody
      • AI325074 antibody
      • AU045576 antibody
      • CDC 21 antibody
      • CDC 54 antibody
      • CDC21 antibody
      • CDC21 homolog antibody
      • CDC21, S. pombe, homolog of antibody
      • CDC54 antibody
      • Cell division cycle 21, S. pombe, homolog of antibody
      • DNA replication licensing factor MCM 4 antibody
      • DNA replication licensing factor MCM4 antibody
      • hCdc 21 antibody
      • hCdc21 antibody
      • Homolog of S. pombe cell devision cycle 21 antibody
      • KIAA4003 antibody
      • mcdc21 antibody
      • MCM 4 antibody
      • MCM 4 minichromosome maintenance deficient 4 antibody
      • mcm4 antibody
      • MCM4 minichromosome maintenance deficient 4 antibody
      • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
      • MCM4_HUMAN antibody
      • Mcmd4 antibody
      • MGC33310 antibody
      • Minichromosome maintenance 4 antibody
      • Minichromosome maintenance complex component 4 antibody
      • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
      • Minichromosome maintenance deficient 4 antibody
      • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
      • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
      • mKIAA4003 antibody
      • NKCD antibody
      • NKGCD antibody
      • P1 CDC21 antibody
      • P1-CDC21 antibody
      • P1CDC21 antibody
      see all


    • All lanes : Anti-MCM4 antibody [EPR5245] (ab124836) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Molt-4 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 97 kDa

    • Flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red (ab124836) or a rabbit IgG in green(negative).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    ab124836 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab124836.
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