Product nameMCM4 overexpression 293T lysate (whole cell)
DescriptionMCM4 overexpression lysate
ab94302 is a 293T cell transfected lysate in which Human MCM4 has been transiently over-expressed using a pCMV-MCM4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundFunction: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Similarity: Belongs to the MCM family. Contains 1 MCM domain.
Our Abpromise guarantee covers the use of ab94302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94302 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-MCM4 antibody (ab88546) at 1/500 dilution
Lane 1 :
MCM4 overexpression 293T lysate (whole cell) (ab94302)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94302 has not yet been referenced specifically in any publications.