Product nameAnti-MCM4 (phospho S54) antibody
See all MCM4 primary antibodies
DescriptionRabbit polyclonal to MCM4 (phospho S54)
Specificityab74014 detects MCM4 only when phosphorylated at serine 54 (Mouse: Ser53).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).
- Extracts from COS-7 cells treated with nocodazole (1uM, 16hours).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab74014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 96 kDa.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
- Information by UniProt
- 19G antibody
- AI325074 antibody
- AU045576 antibody
All lanes : Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours)
Lane 2 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 96 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
ab74014 has not yet been referenced specifically in any publications.