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Anti-MCM4 (phospho S54) antibody (ab74014)

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Western blot - Anti-MCM4 (phospho S54) antibody (ab74014)

    Key features and details

    • Rabbit polyclonal to MCM4 (phospho S54)
    • Suitable for: WB
    • Reacts with: African green monkey
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-MCM4 (phospho S54) antibody
      See all MCM4 primary antibodies
    • Description

      Rabbit polyclonal to MCM4 (phospho S54)
    • Host species

      Rabbit
    • Specificity

      ab74014 detects MCM4 only when phosphorylated at serine 54 (Mouse: Ser53).
    • Tested Applications & Species

      Application Species
      WB
      African green monkey
      See all applications and species data
    • Immunogen

      Synthetic phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).

    • Positive control

      • Extracts from COS-7 cells treated with nocodazole (1uM, 16hours).

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab74014 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    African green monkey
    All applications
    Mouse
    Application Abreviews Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 96 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 96 kDa.

    Target

    • Function

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
    • Sequence similarities

      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P33991 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 17217 Mouse
      • SwissProt: P49717 Mouse
      • Unigene: 1500 Mouse
      • Alternative names

        • 19G antibody
        • AI325074 antibody
        • AU045576 antibody
        • CDC 21 antibody
        • CDC 54 antibody
        • CDC21 antibody
        • CDC21 homolog antibody
        • CDC21, S. pombe, homolog of antibody
        • CDC54 antibody
        • Cell division cycle 21, S. pombe, homolog of antibody
        • DNA replication licensing factor MCM 4 antibody
        • DNA replication licensing factor MCM4 antibody
        • hCdc 21 antibody
        • hCdc21 antibody
        • Homolog of S. pombe cell devision cycle 21 antibody
        • KIAA4003 antibody
        • mcdc21 antibody
        • MCM 4 antibody
        • MCM 4 minichromosome maintenance deficient 4 antibody
        • mcm4 antibody
        • MCM4 minichromosome maintenance deficient 4 antibody
        • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
        • MCM4_HUMAN antibody
        • Mcmd4 antibody
        • MGC33310 antibody
        • Minichromosome maintenance 4 antibody
        • Minichromosome maintenance complex component 4 antibody
        • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
        • Minichromosome maintenance deficient 4 antibody
        • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
        • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
        • mKIAA4003 antibody
        • NKCD antibody
        • NKGCD antibody
        • P1 CDC21 antibody
        • P1-CDC21 antibody
        • P1CDC21 antibody
        see all

      Images

      • Western blot - Anti-MCM4 (phospho S54) antibody (ab74014)
        Western blot - Anti-MCM4 (phospho S54) antibody (ab74014)
        All lanes : Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution

        Lane 1 : Extracts from COS-7 cells
        treated with nocodazole (1uM, 16hours)
        Lane 2 : Extracts from COS-7 cells
        treated with nocodazole (1uM, 16hours) with immunising phosphopeptide at 5 µg

        Lysates/proteins at 5 µg per lane.

        Predicted band size: 96 kDa
        Observed band size: 88 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab74014? Please let us know so that we can cite the reference in this datasheet.

      ab74014 has not yet been referenced specifically in any publications.

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