Anti-MCM4 (phospho S54) antibody (ab74014)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-MCM4 (phospho S54) antibody
See all MCM4 primary antibodies -
Description
Rabbit polyclonal to MCM4 (phospho S54) -
Host species
Rabbit -
Specificity
ab74014 detects MCM4 only when phosphorylated at serine 54 (Mouse: Ser53). -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
-
Immunogen
Synthetic phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).
-
Positive control
- Extracts from COS-7 cells treated with nocodazole (1uM, 16hours).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab74014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 96 kDa. | |
| ELISA | 1/5000. |
Target
-
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4173 Human
- Entrez Gene: 17217 Mouse
- Omim: 602638 Human
- SwissProt: P33991 Human
- SwissProt: P49717 Mouse
- Unigene: 460184 Human
- Unigene: 1500 Mouse
-
Alternative names
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
Images
-
Western blot - Anti-MCM4 (phospho S54) antibody (ab74014)All lanes : Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours)
Lane 2 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 96 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
References
ab74014 has not yet been referenced specifically in any publications.
