Anti-MCM4 (phospho S54) antibody (ab74014)
Key features and details
- Rabbit polyclonal to MCM4 (phospho S54)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
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Product name
Anti-MCM4 (phospho S54) antibody
See all MCM4 primary antibodies -
Description
Rabbit polyclonal to MCM4 (phospho S54) -
Host species
Rabbit -
Specificity
ab74014 detects MCM4 only when phosphorylated at serine 54 (Mouse: Ser53). -
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey
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Immunogen
Synthetic phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).
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Positive control
- Extracts from COS-7 cells treated with nocodazole (1uM, 16hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
African green monkey
|
| All applications |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 96 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 96 kDa. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 17217 Mouse
- SwissProt: P49717 Mouse
- Unigene: 1500 Mouse
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Alternative names
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
Images
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Western blot - Anti-MCM4 (phospho S54) antibody (ab74014)All lanes : Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours)
Lane 2 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 96 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (0)
ab74014 has not yet been referenced specifically in any publications.
