• Product name
    Anti-MCM4 (phospho S54) antibody
    See all MCM4 primary antibodies
  • Description
    Rabbit polyclonal to MCM4 (phospho S54)
  • Host species
  • Specificity
    ab74014 detects MCM4 only when phosphorylated at serine 54 (Mouse: Ser53).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).

  • Positive control
    • Extracts from COS-7 cells treated with nocodazole (1uM, 16hours).


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab74014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 96 kDa.
ELISA 1/5000.


  • Function
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 19G antibody
    • AI325074 antibody
    • AU045576 antibody
    • CDC 21 antibody
    • CDC 54 antibody
    • CDC21 antibody
    • CDC21 homolog antibody
    • CDC21, S. pombe, homolog of antibody
    • CDC54 antibody
    • Cell division cycle 21, S. pombe, homolog of antibody
    • DNA replication licensing factor MCM 4 antibody
    • DNA replication licensing factor MCM4 antibody
    • hCdc 21 antibody
    • hCdc21 antibody
    • Homolog of S. pombe cell devision cycle 21 antibody
    • KIAA4003 antibody
    • mcdc21 antibody
    • MCM 4 antibody
    • MCM 4 minichromosome maintenance deficient 4 antibody
    • mcm4 antibody
    • MCM4 minichromosome maintenance deficient 4 antibody
    • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
    • MCM4_HUMAN antibody
    • Mcmd4 antibody
    • MGC33310 antibody
    • Minichromosome maintenance 4 antibody
    • Minichromosome maintenance complex component 4 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
    • Minichromosome maintenance deficient 4 antibody
    • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
    • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
    • mKIAA4003 antibody
    • NKCD antibody
    • NKGCD antibody
    • P1 CDC21 antibody
    • P1-CDC21 antibody
    • P1CDC21 antibody
    see all


  • All lanes : Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution

    Lane 1 : Extracts from COS-7 cells
    treated with nocodazole (1uM, 16hours)
    Lane 2 : Extracts from COS-7 cells
    treated with nocodazole (1uM, 16hours) with immunising phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 96 kDa
    Observed band size: 88 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.


ab74014 has not yet been referenced specifically in any publications.

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