Anti-MCM5 antibody (ab17967)
- Datasheet
- References (14)
- Protocols
Overview
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Product nameAnti-MCM5 antibody
See all MCM5 primary antibodies -
DescriptionRabbit polyclonal to MCM5
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human, Xenopus laevis
Predicted to work with: Chimpanzee, Orangutan
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Immunogen
Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
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Positive control
- HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab17967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa). | |
| IP | Use a concentration of 5 µg/ml. This antibody is efficient at pulling down the MCM2-7 complex. |
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| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4174 Human
- Entrez Gene: 17218 Mouse
- Omim: 602696 Human
- SwissProt: P33992 Human
- SwissProt: P49718 Mouse
- Unigene: 517582 Human
- Unigene: 5048 Mouse
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Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Images
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Western blot - Anti-MCM5 antibody (ab17967)ab17967, at 0.2
µ g/ml, staining human MCM5 in (3.8, 7.5 or 15µ g for lanes 1, 2 and 3 respectively) HeLa cell lysate by Western blot. ECL detection with 1 minute exposure. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-MCM5 antibody (ab17967)MCM5 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MCM5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17967.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 97kDa; MCM5
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Immunocytochemistry/ Immunofluorescence - Anti-MCM5 antibody (ab17967)ICC/IF image of ab17967 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Kovary KM et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). Read more (PubMed: 29759982) »
- Charrasse S et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). Read more (PubMed: 28785014) »
