Product nameAnti-MCM5 antibody
See all MCM5 primary antibodies
DescriptionRabbit polyclonal to MCM5
Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Xenopus laevis
Predicted to work with: Chimpanzee, Orangutan
Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
- HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab17967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).|
|IP||Use a concentration of 5 µg/ml.
This antibody is efficient at pulling down the MCM2-7 complex.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
- Information by UniProt
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
ab17967, at 0.2
µg/ml, staining human MCM5 in (3.8, 7.5 or 15 µg for lanes 1, 2 and 3 respectively) HeLa cell lysate by Western blot. ECL detection with 1 minute exposure.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB.
MCM5 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MCM5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17967.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 97kDa; MCM5
ICC/IF image of ab17967 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Köhler K et al. The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7. PLoS Biol 17:e2006767 (2019). Read more (PubMed: 30695077) »
- Prikrylova T et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). Read more (PubMed: 31363131) »