Anti-MCM5 antibody (ab17967)
Key features and details
- Rabbit polyclonal to MCM5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MCM5 antibody
See all MCM5 primary antibodies -
Description
Rabbit polyclonal to MCM5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Orangutan
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Immunogen
Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa). |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4174 Human
- Omim: 602696 Human
- SwissProt: P33992 Human
- Unigene: 517582 Human
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Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Images
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Western blot - Anti-MCM5 antibody (ab17967)All lanes : Anti-MCM5 antibody (ab17967) at 0.05 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 82 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab17967 has been referenced in 22 publications.
- Giles KA et al. BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer. Clin Epigenetics 13:37 (2021). PubMed: 33596994
- Corvaisier M et al. The ?-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells. Commun Biol 4:767 (2021). PubMed: 34158617
- Zhang J et al. DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. Nat Commun 11:3951 (2020). PubMed: 32769987
- Ochs F et al. Stabilization of chromatin topology safeguards genome integrity. Nature 574:571-574 (2019). PubMed: 31645724
- Köhler K et al. The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7. PLoS Biol 17:e2006767 (2019). PubMed: 30695077