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    mcm5-antibody-ab17967.pdf

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Anti-MCM5 antibody (ab17967)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (18)

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Western blot - Anti-MCM5 antibody (ab17967)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM5 antibody (ab17967)

Key features and details

  • Rabbit polyclonal to MCM5
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MCM5 antibody
    See all MCM5 primary antibodies
  • Description

    Rabbit polyclonal to MCM5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Chimpanzee
Orangutan
Application Abreviews Notes
WB (4)
1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P33992 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4174 Human
    • Omim: 602696 Human
    • SwissProt: P33992 Human
    • Unigene: 517582 Human
    • Alternative names

      • CDC 46 antibody
      • CDC46 antibody
      • CDC46 homolog antibody
      • Cell division cycle 46 antibody
      • DNA replication licensing factor antibody
      • DNA replication licensing factor MCM 5 antibody
      • DNA replication licensing factor MCM5 antibody
      • MCM 5 antibody
      • MCM5 antibody
      • MCM5_HUMAN antibody
      • MGC5315 antibody
      • Minichromosome maintenance complex component 5 antibody
      • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
      • Minichromosome maintenance deficient 5 antibody
      • Minichromosome maintenance deficient protein 5 antibody
      • P1 CDC46 antibody
      • P1-CDC46 antibody
      see all

    Images

    • Western blot - Anti-MCM5 antibody (ab17967)
      Western blot - Anti-MCM5 antibody (ab17967)

      ab17967, at 0.2µg/ml, staining human MCM5 in (3.8, 7.5 or 15µg for lanes 1, 2 and 3 respectively) HeLa cell lysate by Western blot. ECL detection with 1 minute exposure.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunocytochemistry/ Immunofluorescence - Anti-MCM5 antibody (ab17967)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM5 antibody (ab17967)
      ICC/IF image of ab17967 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (18)

    Publishing research using ab17967? Please let us know so that we can cite the reference in this datasheet.

    ab17967 has been referenced in 18 publications.

    • Köhler K  et al. The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7. PLoS Biol 17:e2006767 (2019). PubMed: 30695077
    • Prikrylova T  et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). PubMed: 31363131
    • Huang J  et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
    • Schade AE  et al. Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB. Oncogene 38:4962-4976 (2019). PubMed: 30833638
    • Kovary KM  et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). PubMed: 29759982
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-MCM5 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - other (Egg)
    Loading amount
    25 µg
    Specification
    Egg
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 03 2012

    Western blot abreview for Anti-MCM5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    50 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 08 2011

    Western blot abreview for Anti-MCM5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    25 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Mirjam Eckert

    Verified customer

    Submitted Oct 29 2009

    Western blot abreview for Anti-MCM5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - other (egg extracts)
    Loading amount
    10 µg
    Specification
    egg extracts
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 12 2008

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