Overview

  • Product name

    Anti-MCM5 antibody [EP2683Y]
    See all MCM5 primary antibodies
  • Description

    Rabbit monoclonal [EP2683Y] to MCM5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MCM5 aa 650 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P33992

  • Positive control

    • HeLa and MCF7 cell lysates; human squamous cell cervical carcinoma tissue; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2683Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IP 1/50.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

Target

  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC 46 antibody
    • CDC46 antibody
    • CDC46 homolog antibody
    • Cell division cycle 46 antibody
    • DNA replication licensing factor antibody
    • DNA replication licensing factor MCM 5 antibody
    • DNA replication licensing factor MCM5 antibody
    • MCM 5 antibody
    • MCM5 antibody
    • MCM5_HUMAN antibody
    • MGC5315 antibody
    • Minichromosome maintenance complex component 5 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
    • Minichromosome maintenance deficient 5 antibody
    • Minichromosome maintenance deficient protein 5 antibody
    • P1 CDC46 antibody
    • P1-CDC46 antibody
    see all

Images

  • All lanes : Anti-MCM5 antibody [EP2683Y] (ab75975) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa

  • ab75975 showing positive staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunofluorescence staining of MCF7 cells with purified ab75975 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • ab75975 at 1/250 dilution staining MCM5 in human squamous cell cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab75975 showing negative staining in Normal prostate tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab75975 showing positive staining in Prostatic carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab75975 showing positive staining in Glioma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab75975 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab75975 showing positive staining in Endometrial carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab75975 showing positive staining in Colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Drissi R  et al. Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks. Cell Cycle 17:2593-2609 (2018). Read more (PubMed: 30516086) »
  • Dilley RL  et al. Break-induced telomere synthesis underlies alternative telomere maintenance. Nature 539:54-58 (2016). Read more (PubMed: 27760120) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 02 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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