Recombinant Anti-MCM5 antibody [EP2683Y] (ab75975)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2683Y] (ab75975)](https://www.abcam.com/ps/products/75/ab75975/Images/ab75975-16-anti-mcm5-antibody-ep2683y-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2683Y] (ab75975)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2683Y] to MCM5
- Suitable for: Flow Cyt (Intra), ICC, ICC/IF, IP, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-MCM5 antibody [EP2683Y]
See all MCM5 primary antibodies -
Description
Rabbit monoclonal [EP2683Y] to MCM5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC, ICC/IF, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7 cell lysate. Mouse spleen lysate; IHC-P: Human tonsil tissue; Flow Cyt (intra): MCF7 cells; ICC: MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2683Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC |
1/50.
For unpurified use at 1/100 - 1/250. |
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
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| WB |
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
For unpurified use at 1/1000 - 1/10000. |
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| IHC-P |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC
1/50. For unpurified use at 1/100 - 1/250. |
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ICC/IF
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). For unpurified use at 1/1000 - 1/10000. |
|
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
Target
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Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4174 Human
- Entrez Gene: 17218 Mouse
- Omim: 602696 Human
- SwissProt: P33992 Human
- SwissProt: P49718 Mouse
- Unigene: 517582 Human
- Unigene: 5048 Mouse
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Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2683Y] (ab75975)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling MCM5 with purified ab75975 at 1/1000 dilution (0.11 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-MCM5 antibody [EP2683Y] (ab75975)](https://www.abcam.com/ps/products/75/ab75975/Images/ab75975-458215-anti-mcm5-antibody-ep2683y-westernblot-human-mouse.jpg)
Western blot - Anti-MCM5 antibody [EP2683Y] (ab75975)All lanes : Anti-MCM5 antibody [EP2683Y] (ab75975) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : Mouse spleen lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 82 kDaBlocking Buffer and concentration: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-MCM5 antibody [EP2683Y] (ab75975)](https://www.abcam.com/ps/products/75/ab75975/Images/ab75975-14-anti-mcm5-antibody-ep2683y-flowcytometry-mcf7-human.jpg)
Flow Cytometry (Intracellular) - Anti-MCM5 antibody [EP2683Y] (ab75975)Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM5 with Purified ab75975 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry - Anti-MCM5 antibody [EP2683Y] (ab75975)](https://www.abcam.com/ps/products/75/ab75975/Images/ab75975-13-anti-mcm5-antibody-ep2683y-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry - Anti-MCM5 antibody [EP2683Y] (ab75975)Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM5 with Purified ab75975 at 1:50 dilution (2.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Anti-MCM5 antibody [EP2683Y] (ab75975)](https://www.abcam.com/ps/products/75/ab75975/Images/ab75975-12-benefits-of-recombinant-antibodies.png)
Anti-MCM5 antibody [EP2683Y] (ab75975)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab75975 has been referenced in 12 publications.
- Lei Y et al. Identification of MCM family as potential therapeutic and prognostic targets for hepatocellular carcinoma based on bioinformatics and experiments. Life Sci 272:119227 (2021). PubMed: 33607151
- Zacarías-Fluck MF et al. The Wnt signaling receptor Fzd9 is essential for Myc-driven tumorigenesis in pancreatic islets. Life Sci Alliance 4:N/A (2021). PubMed: 33653688
- Zhang LL et al. MCM5 Aggravates the HDAC1-Mediated Malignant Progression of Lung Cancer. Front Cell Dev Biol 9:669132 (2021). PubMed: 34409025
- Shao Z et al. RNA Sequence Analyses throughout the Course of Mouse Cardiac Laminopathy Identify Differentially Expressed Genes for Cell Cycle Control and Mitochondrial Function. Sci Rep 10:6632 (2020). PubMed: 32313136
- Niu Y et al. Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins. Onco Targets Ther 13:12015-12025 (2020). PubMed: 33244243