Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2683Y] to MCM5 (Alexa Fluor® 405)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Product nameAnti-MCM5 antibody [EP2683Y] (Alexa Fluor® 405)
See all MCM5 primary antibodies
DescriptionRabbit monoclonal [EP2683Y] to MCM5 (Alexa Fluor® 405)
ConjugationAlexa Fluor® 405. Ex: 402nm, Em: 421nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human MCM5 aa 650 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P33992
- Flow Cyt: HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab210141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
- Information by UniProt
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
Overlay histogram showing HeLa cells stained with ab210141 (red line). The cells were fixed with 80% methanal (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum for 30 min at 22°C. Cells were then incubated with the antibody (ab210141, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Violet laser (405nm) and 450/50 bandpass filter.
ab210141 has not yet been referenced specifically in any publications.