Overview

  • Product name

  • Description

    Rabbit polyclonal to MCM6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, ChIPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MCM6 aa 682-821 (C terminal). The exact sequence is proprietary.
    Database link: Q14566

  • Positive control

    • 293T, A431, HeLa or HepG2 lysate, Human ovarian carcinoma or gastric carcinoma tissue, U2OS cells, HeLa chromatin extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab184147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
ICC/IF 1/1000.
IHC-P 1/100 - 1/1000.
ChIP Use at an assay dependent concentration.

5ug

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

Images

  • All lanes : Anti-MCM6 antibody (ab184147) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : A431 lysate
    Lane 3 : HeLa lysate
    Lane 4 : HepG2 lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 93 kDa



    7.5 % SDS-PAGE

  • Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either normal rabbit IgG (1) or ab184147 (2). The precipitated DNA was detected by PCR with primer set targeting to Myc promoter (1 and 2).

  • Immunofluorescence analysis of 4% paraformaldehyde fixed U2OS cells, labeling MCM6 with ab184147 at a 1/500 dilution (left image), and costained with Hoescht 33342 (right image).

  • Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.

  • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.

References

ab184147 has not yet been referenced specifically in any publications.

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