Key features and details
- Rabbit polyclonal to MCM6
- Suitable for: WB, ICC/IF, IHC-P, ChIP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCM6 antibody
See all MCM6 primary antibodies
DescriptionRabbit polyclonal to MCM6
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, ChIPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human MCM6 aa 682-821 (C terminal). The exact sequence is proprietary.
Database link: Q14566
- 293T, A431, HeLa or HepG2 lysate, Human ovarian carcinoma or gastric carcinoma tissue, U2OS cells, HeLa chromatin extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab184147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 93 kDa.|
|IHC-P||1/100 - 1/1000.|
|ChIP||Use at an assay dependent concentration.
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Binds to chromatin during G1 and detach from it during S phase.
- Information by UniProt
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
All lanes : Anti-MCM6 antibody (ab184147) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : A431 lysate
Lane 3 : HeLa lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 93 kDa
7.5 % SDS-PAGE
Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either normal rabbit IgG (1) or ab184147 (2). The precipitated DNA was detected by PCR with primer set targeting to Myc promoter (1 and 2).
Immunofluorescence analysis of 4% paraformaldehyde fixed U2OS cells, labeling MCM6 with ab184147 at a 1/500 dilution (left image), and costained with Hoescht 33342 (right image).
Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
ab184147 has not yet been referenced specifically in any publications.