Key features and details
- Rabbit polyclonal to MCM6
- Suitable for: IHC-P, IHC-Fr, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MCM6 antibody
See all MCM6 primary antibodies
DescriptionRabbit polyclonal to MCM6
Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Xenopus tropicalis
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 93 kDa.|
|ICC||Use a concentration of 0.5 - 1 µg/ml.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Binds to chromatin during G1 and detach from it during S phase.
- Information by UniProt
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
MCM6 was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190948 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
All lanes : Anti-MCM6 antibody (ab190948) at 0.5 µg/ml
Lane 1 : U87 cell lysate
Lane 2 : COLO320 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : Jurkat cell lysate
Predicted band size: 93 kDa
Immunohistochemistry of paraffin-embedded Human Intestinal Cancer Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.
Immunohistochemistry of paraffin-embedded Rat Brain Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.
Immunocytochemical analysis of HeLa cells, staining MCM6 with ab190948 at 1 µg/ml.
Immunohistochemistry of Frozen section of Human Placenta Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.
ab190948 has been referenced in 2 publications.