Overview

  • Product name

  • Description

    Rabbit polyclonal to MCM6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human MCM6 aa 589-605 (internal sequence).
    Sequence:

    ESEDFIVEQYKHLRQRD


    Database link: Q14566

  • Positive control

    • U87, COLO320, HeLa, MCF7 and Jurkat lysate. Human Intestinal Cancer Tissue, Rat Brain Tissue, and Human Placenta Tissue. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab190948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 93 kDa.
ICC Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

Images

  • MCM6 was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190948 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • All lanes : Anti-MCM6 antibody (ab190948) at 0.5 µg/ml

    Lane 1 : U87 cell lysate
    Lane 2 : COLO320 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate
    Lane 5 : Jurkat cell lysate

    Predicted band size: 93 kDa

  • Immunohistochemistry of paraffin-embedded Human Intestinal Cancer Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.

  • Immunohistochemistry of paraffin-embedded Rat Brain Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.

  • Immunocytochemical analysis of HeLa cells, staining MCM6 with ab190948 at 1 µg/ml.

  • Immunohistochemistry of Frozen section of Human Placenta Tissue labelling MCM6 with ab190948 at 0.5 µg/ml.

References

This product has been referenced in:

See 1 Publication for this product

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