Product nameAnti-MCM6 antibody - C-terminal
See all MCM6 primary antibodies
DescriptionRabbit polyclonal to MCM6 - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
Recombinant fragment within Human MCM6 (C terminal). The exact sequence is proprietary.
Database link: Q14566
- WB: U-2 OS si Ctrl, MEF, INS-1 and DT40 cell lysate. Xenopus lysate. IP: U-2 OS cell lysate. Flow: U-2 OS cells. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Purification notesCrude serum containing sodium azide.
Our Abpromise guarantee covers the use of ab236449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 3 µl to ~400 µg of extract
|WB||1/2000. Predicted molecular weight: 93 kDa.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Binds to chromatin during G1 and detach from it during S phase.
- Information by UniProt
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
All lanes : Anti-MCM6 antibody - C-terminal (ab236449) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) siMCM6
Lane 3 : MEF (mouse embryonic fibroblast cell line) cell lysate
Lane 4 : INS-1 (rat cell line) cell lysate
Lane 5 : DT40 (chicken lymphoma cell line) cell lysate
Lane 6 : Xenopus lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 93 kDa
Block: TBS-T 5% milk. Incubated overnight with primary antibody.
Treated with control or MCM6 siRNA to show specificity.
Top panel: U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for MCM6 using ab236449 at a dilution of 1/2000 in ICC/IF.
Middle panel: DAPI.
Bottom panel: Merged image.
Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.
Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling MCM6 with ab236449 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).
Cells were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.
MCM6 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236449.
Lane 1: U-2 OS whole cell lysate (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236449 in U-2 OS whole cell lysate.
For western blotting ab236449 was used at a 1/2000 dilution.
ab236449 has not yet been referenced specifically in any publications.