Key features and details
- Rabbit polyclonal to MCM6 - C-terminal
- Suitable for: ICC/IF, IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
- Isotype: IgG
Product nameAnti-MCM6 antibody - C-terminal
See all MCM6 primary antibodies
DescriptionRabbit polyclonal to MCM6 - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
Recombinant fragment within Human MCM6 (C terminal). The exact sequence is proprietary.
Database link: Q14566
- WB: U-2 OS si Ctrl, MEF, INS-1 and DT40 cell lysate. Xenopus laevis lysate. IP: U-2 OS cell lysate. Flow Cyt: U-2 OS cells. ICC: U-2 OS cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Purification notesCrude serum containing sodium azide.
Our Abpromise guarantee covers the use of ab236449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 3 µl to ~400 µg of extract
|WB||1/2000. Predicted molecular weight: 93 kDa.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Binds to chromatin during G1 and detach from it during S phase.
- Information by UniProt
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
All lanes : Anti-MCM6 antibody - C-terminal (ab236449) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) siMCM6
Lane 3 : MEF (mouse embryonic fibroblast cell line) cell lysate
Lane 4 : INS-1 (rat cell line) cell lysate
Lane 5 : DT40 (chicken lymphoma cell line) cell lysate
Lane 6 : Xenopus laevis lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 93 kDa
Block: TBS-T 5% milk. Incubated overnight with primary antibody.
Treated with control or MCM6 siRNA to show specificity.
Top panel: U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for MCM6 using ab236449 at a dilution of 1/2000 in ICC/IF.
Middle panel: DAPI.
Bottom panel: Merged image.
Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.
Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling MCM6 with ab236449 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).
Cells were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.
MCM6 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236449.
Lane 1: U-2 OS whole cell lysate (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236449 in U-2 OS whole cell lysate.
For western blotting ab236449 was used at a 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236449 has not yet been referenced specifically in any publications.