Anti-MCM6 antibody - C-terminal (ab236449)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MCM6 antibody - C-terminal
See all MCM6 primary antibodies -
Description
Rabbit polyclonal to MCM6 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis -
Immunogen
Recombinant fragment within Human MCM6 (C terminal). The exact sequence is proprietary.
Database link: Q14566 -
Positive control
- WB: U-2 OS si Ctrl, MEF, INS-1 and DT40 cell lysate. Xenopus lysate. IP: U-2 OS cell lysate. Flow: U-2 OS cells. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Purity
Whole antiserum -
Purification notes
Crude serum containing sodium azide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/2000. | |
| IP | Use at an assay dependent concentration. Use 3 µl to ~400 µg of extract |
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| Flow Cyt | 1/200. | |
| WB | 1/2000. Predicted molecular weight: 93 kDa. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Binds to chromatin during G1 and detach from it during S phase. - Information by UniProt
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Database links
- Entrez Gene: 4175 Human
- Entrez Gene: 17219 Mouse
- Entrez Gene: 29685 Rat
- Entrez Gene: 398071 Xenopus laevis
- Omim: 601806 Human
- SwissProt: Q14566 Human
- SwissProt: P97311 Mouse
- SwissProt: Q62724 Rat
see all -
Alternative names
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
see all
Images
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Western blot - Anti-MCM6 antibody - C-terminal (ab236449)All lanes : Anti-MCM6 antibody - C-terminal (ab236449) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) siMCM6
Lane 3 : MEF (mouse embryonic fibroblast cell line) cell lysate
Lane 4 : INS-1 (rat cell line) cell lysate
Lane 5 : DT40 (chicken lymphoma cell line) cell lysate
Lane 6 : Xenopus lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 93 kDaBlock: TBS-T 5% milk. Incubated overnight with primary antibody.
Treated with control or MCM6 siRNA to show specificity.
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Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody - C-terminal (ab236449)Top panel: U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for MCM6 using ab236449 at a dilution of 1/2000 in ICC/IF.
Middle panel: DAPI.
Bottom panel: Merged image.
Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.
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Flow Cytometry - Anti-MCM6 antibody - C-terminal (ab236449)Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling MCM6 with ab236449 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).
Cells were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.
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Immunoprecipitation - Anti-MCM6 antibody - C-terminal (ab236449)MCM6 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236449.
Lane 1: U-2 OS whole cell lysate (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236449 in U-2 OS whole cell lysate.
For western blotting ab236449 was used at a 1/2000 dilution.
References
ab236449 has not yet been referenced specifically in any publications.
