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    mcm6-antibody-c-terminal-ab236449.pdf

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Anti-MCM6 antibody - C-terminal (ab236449)

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Western blot - Anti-MCM6 antibody - C-terminal (ab236449)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody - C-terminal (ab236449)
  • Flow Cytometry - Anti-MCM6 antibody - C-terminal (ab236449)
  • Immunoprecipitation - Anti-MCM6 antibody - C-terminal (ab236449)

Key features and details

  • Rabbit polyclonal to MCM6 - C-terminal
  • Suitable for: ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
  • Isotype: IgG

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Protein
Product image
Recombinant Human MCM6 protein (ab114758)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MCM6 antibody - C-terminal
    See all MCM6 primary antibodies
  • Description

    Rabbit polyclonal to MCM6 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human MCM6 (C terminal). The exact sequence is proprietary.
    Database link: Q14566

  • Positive control

    • WB: U-2 OS si Ctrl, MEF, INS-1 and DT40 cell lysate. Xenopus laevis lysate. IP: U-2 OS cell lysate. Flow Cyt: U-2 OS cells. ICC: U-2 OS cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Purification notes

    Crude serum containing sodium azide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human MCM6 protein (ab114758)
  • Related Products

    • Recombinant Human MCM6 protein (ab114758)

Applications

Our Abpromise guarantee covers the use of ab236449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000.
IP Use at an assay dependent concentration.

Use 3 µl to ~400 µg of extract

Flow Cyt 1/200.
WB 1/2000. Predicted molecular weight: 93 kDa.

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Target information above from: UniProt accession Q14566 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4175 Human
    • Entrez Gene: 17219 Mouse
    • Entrez Gene: 29685 Rat
    • Entrez Gene: 398071 Xenopus laevis
    • Omim: 601806 Human
    • SwissProt: Q14566 Human
    • SwissProt: P97311 Mouse
    • SwissProt: Q62724 Rat
    • SwissProt: Q7ZY18 Xenopus laevis
    • Unigene: 444118 Human
    • Unigene: 4933 Mouse
    • Unigene: 33226 Rat
    see all
  • Alternative names

    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

Images

  • Western blot - Anti-MCM6 antibody - C-terminal (ab236449)
    Western blot - Anti-MCM6 antibody - C-terminal (ab236449)
    All lanes : Anti-MCM6 antibody - C-terminal (ab236449) at 1/2000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl
    Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) siMCM6
    Lane 3 : MEF (mouse embryonic fibroblast cell line) cell lysate
    Lane 4 : INS-1 (rat cell line) cell lysate
    Lane 5 : DT40 (chicken lymphoma cell line) cell lysate
    Lane 6 : Xenopus laevis lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 93 kDa



    Block: TBS-T 5% milk. Incubated overnight with primary antibody.

    Treated with control or MCM6 siRNA to show specificity.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody - C-terminal (ab236449)
    Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody - C-terminal (ab236449)

    Top panel: U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for MCM6 using ab236449 at a dilution of 1/2000 in ICC/IF.

    Middle panel: DAPI.

    Bottom panel: Merged image.

    Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.

  • Flow Cytometry - Anti-MCM6 antibody - C-terminal (ab236449)
    Flow Cytometry - Anti-MCM6 antibody - C-terminal (ab236449)

    Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling MCM6 with ab236449 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).

    Cells  were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.

  • Immunoprecipitation - Anti-MCM6 antibody - C-terminal (ab236449)
    Immunoprecipitation - Anti-MCM6 antibody - C-terminal (ab236449)

    MCM6 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236449.

    Lane 1: U-2 OS whole cell lysate (Input).

    Lane 2: Control IgG in U-2 OS whole cell lysate.

    Lane 3: ab236449 in U-2 OS whole cell lysate.

    For western blotting ab236449 was used at a 1/2000 dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab236449? Please let us know so that we can cite the reference in this datasheet.

    ab236449 has not yet been referenced specifically in any publications.

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