Product nameAnti-MCM7 antibody [47DC141]
See all MCM7 primary antibodies
DescriptionMouse monoclonal [47DC141] to MCM7
Tested applicationsSuitable for: IHC-P, IP, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Xenopus laevis
Recombinant full length protein (Human).
- Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Light chain typeunknown
Our Abpromise guarantee covers the use of ab2360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.|
|IP||Use at 2 µg/mg of lysate.|
|WB||1/25 - 1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Incubate for 2 hrs at RT for colorimetric detection, can dilute more with ECL+ and with overnight incubation. By Western blot, this antibody detects a band of 80 kDa, which corresponds to the predicted molecular weight of Cdc47 / MCM7.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
All lanes : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilution
Lane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.
Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.
All lanes : HRP conjugated Donkey anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab2360 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.
This image shows immunostaining of rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC. Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.
The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.
ab2360 staining MCM7 in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.
Lane 1 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/50 dilution
Lane 2 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilution
Lane 3 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/500 dilution
All lanes : Whole cell lysate prepared from SW780 cells
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Gel run under denaturing conditions 4-12% gradient.
ICC/IF image of ab2360 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HeLA cells stained with ab2360 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab2360 staining MCM7 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.
This product has been referenced in:
- Kovary KM et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). Read more (PubMed: 29759982) »
- Charrasse S et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). Read more (PubMed: 28785014) »