Product nameAnti-MCM7/PRL antibody [47DC141]
See all MCM7/PRL primary antibodies
DescriptionMouse monoclonal [47DC141] to MCM7/PRL
Tested applicationsSuitable for: IHC-P, IP, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Xenopus laevis
Recombinant full length protein corresponding to Human MCM7/PRL.
- Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Light chain typeunknown
Our Abpromise guarantee covers the use of ab2360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.|
|IP||Use at 2 µg/mg of lysate.|
|WB||1/25 - 1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
Incubate for 2 hrs at RT for colorimetric detection, can dilute more with ECL+ and with overnight incubation. By Western blot, this antibody detects a band of 80 kDa, which corresponds to the predicted molecular weight of Cdc47 / MCM7/PRL.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
All lanes : Anti-MCM7/PRL antibody [47DC141] (ab2360) at 1/200 dilution
Lane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.
Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.
All lanes : HRP conjugated Donkey anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab2360 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with MCM7/PRL, using ABC and DAB chromagen.
This image shows immunostaining of MCM7/PRL in rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody MCM7/PRL (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC. Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.
The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.
ab2360 staining MCM7/PRL in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.
Lane 1 : Anti-MCM7/PRL antibody [47DC141] (ab2360) at 1/50 dilution
Lane 2 : Anti-MCM7/PRL antibody [47DC141] (ab2360) at 1/200 dilution
Lane 3 : Anti-MCM7/PRL antibody [47DC141] (ab2360) at 1/500 dilution
All lanes : Whole cell lysate prepared from SW780 cells
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Gel run under denaturing conditions 4-12% gradient.
ICC/IF image of MCM7/PRL (ab2360) stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HeLA cells stained with MCM7/PRL (ab2360) (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab2360 staining MCM7/PRL in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.
This product has been referenced in:
- Kovary KM et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). Read more (PubMed: 29759982) »
- Charrasse S et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). Read more (PubMed: 28785014) »