Key features and details
- Rabbit polyclonal to MCM7/PRL
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCM7/PRL antibody
See all MCM7/PRL primary antibodies
DescriptionRabbit polyclonal to MCM7/PRL
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human MCM7/PRL aa 360-719. NP_005907.3
GGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGS SGVGLTAAVLRDSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIH EVMEQQTISIAKAGILTTLNARCSILAAANPAYGRYNPRRSLEQNIQLPA ALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPSQFEPLDMKLM RRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAI LRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPAD VIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQV NASRTRITFV
Database link: P33993-1
- WB: A549, HeLa, HL-60, MCF7 and SW480 cell extracts. ICC/IF: HeLa cells.
This product was previously labelled as MCM7
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab238529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 81 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
All lanes : Anti-MCM7/PRL antibody (ab238529) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) cell extracts
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) cell extracts
Lane 5 : SW480 (Human colorectal adenocarcinoma cell line) cell extracts
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 10 seconds
Blocking buffer: 3% non-fat dry milk in TBST.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for MCM7/PRL using ab238529 (Top panel, green) at a 1/50 dilution in ICC/IF. Counterstained with DAPI (Bottom panel, merge).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238529 has not yet been referenced specifically in any publications.