Product nameAnti-MCM7/PRL antibody
See all MCM7/PRL primary antibodies
DescriptionRabbit polyclonal to MCM7/PRL
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human MCM7/PRL aa 360-719. NP_005907.3
GGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGS SGVGLTAAVLRDSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIH EVMEQQTISIAKAGILTTLNARCSILAAANPAYGRYNPRRSLEQNIQLPA ALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPSQFEPLDMKLM RRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAI LRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPAD VIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQV NASRTRITFV
Database link: P33993-1
- WB: A549, HeLa, HL-60, MCF7 and SW480 cell extracts. ICC/IF: HeLa cells.
This product was previously labelled as MCM7
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab238529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 81 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
All lanes : Anti-MCM7/PRL antibody (ab238529) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) cell extracts
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) cell extracts
Lane 5 : SW480 (Human colorectal adenocarcinoma cell line) cell extracts
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 10 seconds
Blocking buffer: 3% non-fat dry milk in TBST.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for MCM7/PRL using ab238529 (Top panel, green) at a 1/50 dilution in ICC/IF. Counterstained with DAPI (Bottom panel, merge).
ab238529 has not yet been referenced specifically in any publications.