Overview

  • Product name

  • Description

    Rabbit polyclonal to MCM7/PRL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human MCM7/PRL aa 500-550. The exact sequence is proprietary. NP_005907.3
    Database link: P33993

  • Positive control

    • WB: HeLa and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as MCM7

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245375 was affinity purified using an epitope specific to MCM7/PRL immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 81 kDa.

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDABP0042 antibody
    • CDC 47 antibody
    • CDC47 antibody
    • CDC47 homolog antibody
    • Cdc47, S. cerevisiae, homolog of antibody
    • DNA replication licensing factor MCM7 antibody
    • Homolog of S. cerevisiae Cdc47 antibody
    • MCM 2 antibody
    • MCM 7 antibody
    • MCM2 antibody
    • MCM2, formerly antibody
    • Mcm7 antibody
    • MCM7 minichromosome maintenance deficient 7 antibody
    • MCM7_HUMAN antibody
    • Minichromosome Maintainence 7 antibody
    • Minichromosome maintainence, S. cerevisiae, homolog of antibody
    • Minichromosome maintenance complex component 7 antibody
    • Minichromosome maintenance deficient 7 antibody
    • Minichromosome maintenance protein 7 antibody
    • P1.1 MCM3 antibody
    • P1.1-MCM3 antibody
    • P1CDC47 antibody
    • P85MCM antibody
    • PNAS 146 antibody
    • PNAS146 antibody
    • PPP1R104 antibody
    • Protein phosphatase 1, regulatory subunit 104 antibody
    see all

Images

  • All lanes : Anti-MCM7/PRL antibody (ab245375) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 81 kDa


    Exposure time: 30 seconds
  • MCM7/PRL was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245375 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245375 IP in HeLa whole cell lysate.
    Lane 2: Control IgG.

    Chemiluminescence detection: 10 seconds.

References

ab245375 has not yet been referenced specifically in any publications.

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