Key features and details
- Rabbit polyclonal to MCM7/PRL
- Suitable for: ChIP, WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCM7/PRL antibody
See all MCM7/PRL primary antibodies
DescriptionRabbit polyclonal to MCM7/PRL
Tested applicationsSuitable for: ChIP, WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
Recombinant fragment corresponding to Human MCM7/PRL aa 14-275.
Database link: P33993
- WB: H1299 cell lysate. IHC-P: Cal27 Xenograft tissue. ICC/IF: HeLa cells. IP: HeLa cells. ChIP: HeLa cells.
This product was previously labelled as MCM7
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 81 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/250.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96849 at 1/200 dilution (1).Merged with DNA probe (2).
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96849 at 1/100 dilution.
MCM7 was immunoprecipitated from 40 μg of HeLa whole cell lysate with ab96849 at 1/1000 dilution. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1: 40 μg HeLa whole cell extract (Input).
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal.
7.5 % SDS-PAGE
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
ab96849 has been referenced in 1 publication.
- Miotto B et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . PubMed: 24726359