Key features and details
- Rabbit polyclonal to MCM7/PRL - BSA and Azide free
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Hamster, Human
- Isotype: IgG
Product nameAnti-MCM7/PRL antibody - BSA and Azide free
See all MCM7/PRL primary antibodies
DescriptionRabbit polyclonal to MCM7/PRL - BSA and Azide free
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Hamster, Human
Predicted to work with: Cow
Recombinant fragment (His-tag) corresponding to Human MCM7/PRL aa 562-719 (C terminal).
YIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAILR LSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADVI FATVRELVS GGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
Database link: P33993
- Human HeLa, SiHa, C33A, WI38 cell extracts; Mouse NIH 3T3 cell extract.
This product was previously labelled as MCM7
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: 50% Glycerol, 50% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab179904 is filter-sterilised.
Our Abpromise guarantee covers the use of ab179904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 81 kDa.|
|ICC/IF||1/200 - 1/1000.|
|IP||Use at an assay dependent concentration.|
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
All lanes : Anti-MCM7/PRL antibody - BSA and Azide free (ab179904) at 1/1000 dilution
Lane 1 : SiHa whole cell extract
Lane 2 : C33A whole cell extract
Lane 3 : WI38 whole cell extract
Lysates/proteins at 100000 cells per lane.
Predicted band size: 81 kDa
All lanes : Anti-MCM7/PRL antibody - BSA and Azide free (ab179904) at 1/2000 dilution
Lane 1 : HeLa whole cell extract
Lane 2 : HeLa whole cell extract treated with 100 nM adriamycin for 24 hours
Lane 3 : NIH 3T3 whole cell extract
Predicted band size: 81 kDa
Immunoprecipitation analysis on crude extracts of fibroblast cell line WI38 using ab179904.
Lane 1; Immunoprecipitation with pre-immune serum.
Lane 2; Immnoprecipitation with ab179904 antiserum.
Cells were labeled with S35 methionine and MCM7/PRL was immunoprecipitated with ab179904 followed by SDS-PAGE and autoradiography.
Immunofluorecent staining and confocal microscopic analysis of formaldehyde-fixed G1 phase HeLa cell nucleus, labeling MCM7/PRL using ab179904 at 1/200 dilution, after treatment with protein cross-linking reagent, DSP and chromatin extraction.
ab179904 has been referenced in 3 publications.
- Fujita M et al. Nuclear organization of DNA replication initiation proteins in mammalian cells. J Biol Chem 277:10354-61 (2002). PubMed: 11779870
- Fujita M et al. In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP. J Biol Chem 272:10928-35 (1997). PubMed: 9099751
- Fujita M et al. hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase. J Biol Chem 271:4349-54 (1996). PubMed: 8626784