Recombinant Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MCM7/PRL antibody [EPR1973Y]
See all MCM7/PRL primary antibodies -
Description
Rabbit monoclonal [EPR1973Y] to MCM7/PRL -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MCM7/PRL aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa and Jurkat cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as MCM7
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1973Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Predicted molecular weight: 81 kDa. | |
| Flow Cyt | 1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/100 - 1/250. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4176 Human
- Omim: 600592 Human
- SwissProt: P33993 Human
- Unigene: 438720 Human
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Alternative names
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
Images
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![Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)](https://www.abcam.com/ps/products/134/ab134194/Images/ab134194-148526-anti-mcm7-antibody-epr1973y-western-blot.jpg)
Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)All lanes : Anti-MCM7/PRL antibody [EPR1973Y] (ab134194) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 81 kDa
Protocols
Datasheets and documents
References
ab134194 has not yet been referenced specifically in any publications.
