Recombinant Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)
![Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)](https://www.abcam.com/ps/products/134/ab134194/Images/ab134194-148526-anti-mcm7-antibody-epr1973y-western-blot.jpg "Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1973Y] to MCM7/PRL
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-MCM7/PRL antibody [EPR1973Y]
See all MCM7/PRL primary antibodies -
Description
Rabbit monoclonal [EPR1973Y] to MCM7/PRL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human MCM7/PRL aa 1-100. The exact sequence is proprietary.
-
Positive control
- WB: HeLa and Jurkat cell lysates. Flow Cyt: HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as MCM7.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1973Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 81 kDa.
|
|
| Flow Cyt |
1/600.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 81 kDa. |
|
Flow Cyt
1/600. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4176 Human
- Omim: 600592 Human
- SwissProt: P33993 Human
- Unigene: 438720 Human
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Alternative names
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
Images
-
Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)All lanes : Anti-MCM7/PRL antibody [EPR1973Y] (ab134194) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 81 kDa -
![Flow Cytometry - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)](https://www.abcam.com/ps/products/134/ab134194/Images/ab134194-4-anti-mcm7-prl-antibody-epr1973-flowcytometry-hela-human.jpg)
Flow Cytometry - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MCM7/PRL with purified ab134194 at 1/600 dilution (1µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). -
![Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)](https://www.abcam.com/ps/products/134/ab134194/Images/ab134194-2-benefits-of-recombinant-antibodies.png)
Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)
Protocols
Datasheets and documents
References (0)
ab134194 has not yet been referenced specifically in any publications.
