Overview

  • Product name
    Anti-MCP1 antibody [2H5]
    See all MCP1 primary antibodies
  • Description
    Armenian Hamster monoclonal [2H5] to MCP1
  • Host species
    Armenian hamster
  • Specificity
    This antibody is specific to mouse MCP-1.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    CHO expressed, recombinant mouse MCP-1.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab21396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 5 - 10 µg/ml. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.
Flow Cyt Use 0.5µg for 106 cells.

in 100µl volume for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP1 producing cells within mixed cell populations.

 

 

 

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
IHC-FoFr Use at an assay dependent concentration. Samples need to be saponin-treated.

Target

  • Function
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Images

  • ELISA - Anti-MCP1 antibody [2H5] (ab21396)
    ELISA - Anti-MCP1 antibody [2H5] (ab21396)
    Ab21396 staining human MCP-1 by ELISA. Image shows a standard curve obtained with increasing concentrations of human MCP-1.

References

This product has been referenced in:
  • Yang SY  et al. Downregulation of angiotensin type 1 receptor and nuclear factor-?B by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction. Sci Rep 6:33705 (2016). Read more (PubMed: 27659793) »
  • Li X  et al. Calcium Oxalate Induces Renal Injury through Calcium-Sensing Receptor. Oxid Med Cell Longev 2016:5203801 (2016). Read more (PubMed: 27965733) »

See all 5 Publications for this product

Publishing research using ab21396? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

9 reviews or Q&A

10
Votes: Highest First

Reference using ab21396 in IHC-Fr

Below is a link to a paper that uses clone 2H5 (same clone as ab21396) for acetone fixed, frozen tissue staining (Figure 8).

http://ajprenal.physiology.org/content/280/2/F343

This customer already used ab21396 (Anti-MCP1 antibody [2H5]) to conducted the wb with rat bladder tissue sample, and the results shows strong signal at 25kDa, and the references also show in the 25kDa, however, our website datasheet indicated the pred...

Read More

Thank you for contacting us.

As shown on the following SwissProtlink, http://www.uniprot.org/uniprot/P14844, the calculated molecular weight of the Rat MCP1 is 16.4 kDa, not 11 kDa as for Human. The differences in the molecular weight observed...

Read More

1) Wo bekomme ich einen sekundaeren AK fuer Armenian Hamster her? WB, FlowCyt, ICC/IF?
2) Gibts den auch Fluorochrom-konjugiert?

Vielen Dank für Ihre Anrufe.
Wie besprochen können wir den MCP1 Antikörper für Western blot und Durchflußzytometrie garantieren (www.abcam.com/Abpromise). In der Immuncytochemie/Immunfluoreszenz (ICC/IF) wurde er noch nicht getestet, aber es beste...

Read More

ELISA abreview for Anti-MCP1 antibody [2H5]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (aotic endothelial cells)
Specification
aotic endothelial cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type
Indirect
Read More
Username

Abcam user community

Verified customer

Submitted Jan 31 2012

Thank you for your help.The translation: Human umbilical cord endothelial cells and smooth muscle cells in rats are right.And the customerconfirmed that MCP-1 is indeed expressed by these tissues,but they didnot get good results with the ab21396 which ...

Read More

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. I am glad to learn that the customer expects expression of the protein in these tissues, because following good scientific practise a posit...

Read More

Dear scientist: My customer used ab21396 ( Lot. #GR37-1 ) in WB assay, but they didn't get a satisfactory result. The details listed as below. 1. Order details: · Batch No: GR37-1 · Purchase No or order No: PO#110914-1 and order no: 949...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this Anti-MCP1 antibody. We have received no complaints about this product therefore I a...

Read More

I want to check that if the product ab21396 can be done mmunohistology on paraffin tissues? Thank you. Regards. Looking forwards to hearing from you soon.

Thank you for your enquiry. I have checked with our laboratory and it seems that this product has not been tested in paraffin embedded sections. The application that we have stated on the datasheet refers to frozen sections, and tissue/cells grown ...

Read More

Customer needs IHC-frozen protocol with saponin treatment. Rat retina fixation protocol and secondary.

Thank you for your enquiry. The following paper has a brief description of a procedure for staining frozen sections of rat retina with MCP-1 antibody. It is not the same antibody as our hamster antibody but the procedure is standard. The important ...

Read More

Thank you for your responses before. I am sorry for my first unclear enquiry. I am doing immunohistochemistry ,so I just want a IHC postive control, and what tissue is the positve control in my experiment. Thanks a lot again!

Thank you for your reply. A MCP-1 positive control in tissue is a bit tricky as production usually tracks with disease states (for example trauma or autoimmune disease). One of the best positive controls for MCP-1 in tissue is the central nervous ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up