Anti-MCP1 antibody [2H5] (ab21396)
Key features and details
- Armenian hamster monoclonal [2H5] to MCP1
- Suitable for: ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-MCP1 antibody [2H5]
See all MCP1 primary antibodies -
Description
Armenian hamster monoclonal [2H5] to MCP1 -
Host species
Armenian hamster -
Specificity
This antibody is specific to mouse MCP-1. -
Tested Applications & Species
Application Species ELISA Recombinant fragment -
Immunogen
CHO expressed, recombinant mouse MCP-1.
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Positive control
- Purchase matching WB positive control:Recombinant human MCP1 protein
- IHC- central nervous system of rats or mice with experimental autoimmune encephalomyelitis.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
2H5 -
Isotype
IgG -
Research areas
Associated products
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ELISA |
Recombinant fragment
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Application | Abreviews | Notes |
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ELISA | (1) |
Use a concentration of 5 - 10 µg/ml. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.
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Notes |
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ELISA
Use a concentration of 5 - 10 µg/ml. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. |
Target
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab21396 has been referenced in 10 publications.
- Giri J et al. CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury. Cell Rep 30:1923-1934.e4 (2020). PubMed: 32049021
- Yang X et al. Metformin prevents nephrolithiasis formation by inhibiting the expression of OPN and MCP-1 in vitro and in vivo. Int J Mol Med 43:1611-1622 (2019). PubMed: 30720053
- Lai XL et al. Apc gene suppresses intracranial aneurysm formation and rupture through inhibiting the NF-?B signaling pathway mediated inflammatory response. Biosci Rep 39:N/A (2019). PubMed: 30808715
- Yang SY et al. Downregulation of angiotensin type 1 receptor and nuclear factor-?B by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction. Sci Rep 6:33705 (2016). PubMed: 27659793
- Li X et al. Calcium Oxalate Induces Renal Injury through Calcium-Sensing Receptor. Oxid Med Cell Longev 2016:5203801 (2016). PubMed: 27965733
- Zhang S et al. Comparison of various niches for endothelial progenitor cell therapy on ischemic myocardial repair: coexistence of host collateralization and Akt-mediated angiogenesis produces a superior microenvironment. Arterioscler Thromb Vasc Biol 32:910-23 (2012). PubMed: 22328781
- Negishi T et al. Diphenylarsinic acid increased the synthesis and release of neuroactive and vasoactive peptides in rat cerebellar astrocytes. J Neuropathol Exp Neurol 71:468-79 (2012). PubMed: 22588385
- Zhang Y et al. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol 168:3088-98 (2002). PubMed: 11884483
- Luo Y et al. Chemokine amplification in mesangial cells. J Immunol 163:3985-92 (1999). PubMed: 10491001
- Luo Y et al. Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1. J Immunol 153:3708-16 (1994). Inhibition Assay . PubMed: 7523503