Overview

  • Product name
    Anti-MCP1 antibody [2H5]
    See all MCP1 primary antibodies
  • Description
    Armenian Hamster monoclonal [2H5] to MCP1
  • Host species
    Armenian hamster
  • Specificity
    This antibody is specific to mouse MCP-1.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    CHO expressed, recombinant mouse MCP-1.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab21396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 5 - 10 µg/ml. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.
Flow Cyt Use 0.5µg for 106 cells.

in 100µl volume for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP1 producing cells within mixed cell populations.

 

 

 

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
IHC-FoFr Use at an assay dependent concentration. Samples need to be saponin-treated.

Target

  • Function
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Images

  • Ab21396 staining human MCP-1 by ELISA. Image shows a standard curve obtained with increasing concentrations of human MCP-1.

References

This product has been referenced in:
  • Yang SY  et al. Downregulation of angiotensin type 1 receptor and nuclear factor-?B by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction. Sci Rep 6:33705 (2016). Read more (PubMed: 27659793) »
  • Li X  et al. Calcium Oxalate Induces Renal Injury through Calcium-Sensing Receptor. Oxid Med Cell Longev 2016:5203801 (2016). Read more (PubMed: 27965733) »
See all 6 Publications for this product

Customer reviews and Q&As

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (aotic endothelial cells)
Specification
aotic endothelial cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type
Indirect

Abcam user community

Verified customer

Submitted Jan 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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