Anti-MCP1 antibody (ab25124)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: WB, Neutralising, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-MCP1 antibody
See all MCP1 primary antibodies -
Description
Rabbit polyclonal to MCP1 -
Host species
Rabbit -
Specificity
ab25124 reacts with both rat MCP1 and its mouse homolog JE. -
Tested applications
Suitable for: WB, Neutralising, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Rat MCP1 aa 1-73 (N terminal).
Sequence:MQVSVTLLGL LFTVAACSIH VLSQPDAVNA PLTCCYSFTG KMIPMSRLEN YKRITSSRCP KEAVVFVTKL KRE
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration.
|
| Neutralising |
Use at an assay dependent concentration.
|
|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
Used at a dilution of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information). ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
|
Neutralising
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. Used at a dilution of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information). ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 20296 Mouse
- Entrez Gene: 24770 Rat
- SwissProt: P10148 Mouse
- SwissProt: P14844 Rat
- Unigene: 290320 Mouse
- Unigene: 4772 Rat
-
Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (130)
ab25124 has been referenced in 130 publications.
- Hou C et al. Mono-macrophage-Derived MANF Protects Against Lipopolysaccharide-Induced Acute Kidney Injury via Inhibiting Inflammation and Renal M1 Macrophages. Inflammation 44:693-703 (2021). PubMed: 33145627
- Li Z et al. SPATA4 improves aging-induced metabolic dysfunction through promotion of preadipocyte differentiation and adipose tissue expansion. Aging Cell 20:e13282 (2021). PubMed: 33314576
- Li X et al. Wnt5a promotes renal tubular inflammation in diabetic nephropathy by binding to CD146 through noncanonical Wnt signaling. Cell Death Dis 12:92 (2021). PubMed: 33462195
- Huang J et al. Role of tubular epithelial arginase-II in renal inflammaging. NPJ Aging Mech Dis 7:5 (2021). PubMed: 33654066
- Wang C et al. Simultaneous targeting of oxidative stress and fibrosis reverses cardiomyopathy-induced ventricular remodelling and dysfunction. Br J Pharmacol 178:2424-2442 (2021). PubMed: 33660265