Anti-MCP1 antibody (ab25124)
Rabbit polyclonal MCP1 antibody. Validated in WB, IHC, Neut, Flow Cyt and tested in Mouse, Rat. Cited in 42 publication(s). Independently reviewed in 5 review(s).
- Datasheet
- References (48)
- Protocols
Overview
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Product nameAnti-MCP1 antibody
See all MCP1 primary antibodies -
DescriptionRabbit polyclonal to MCP1
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Host speciesRabbit
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Specificityab25124 reacts with both rat MCP1 and its mouse homolog JE.
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Tested applicationsSuitable for: IHC-P, Flow Cyt, Neutralising, WBmore details
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Species reactivityReacts with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Rat MCP1 aa 1-73 (N terminal).
Sequence:MQVSVTLLGL LFTVAACSIH VLSQPDAVNA PLTCCYSFTG KMIPMSRLEN YKRITSSRCP KEAVVFVTKL KRE
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Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Rat Kidney.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab25124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/100. Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information). ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| Neutralising | Use at an assay dependent concentration. | |
| WB | 1/2000. Detects a band of approximately 25 kDa. |
Target
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FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
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Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
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Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 20296 Mouse
- Entrez Gene: 24770 Rat
- SwissProt: P10148 Mouse
- SwissProt: P14844 Rat
- Unigene: 290320 Mouse
- Unigene: 4772 Rat
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (ab25124)IHC image of MCP1 staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25124, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-MCP1 antibody (ab25124)All lanes : Anti-MCP1 antibody (ab25124) at 1/1000 dilution
Lane 1 : 40ug whole rat spinal cord protein lysate
Lane 2 : 40ug whole mouse spinal cord protein lysate
Lane 3 : Naive Rat
Lane 4 : Sham 1
Lane 5 : Sham 2
Lane 6 : Injured 1
Lane 7 : Injured 2
Lane 8 : Injured 3
Secondary
All lanes : Goat anti-Rabbit HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 7 December 2005.
References
This product has been referenced in:
- Zlotkowska A & Andronowska A Variable chemokine expression in porcine trophoblasts and endometrium during the peri-implantation period. Theriogenology 131:16-27 (2019). Read more (PubMed: 30928625) »
- Yang X et al. Metformin prevents nephrolithiasis formation by inhibiting the expression of OPN and MCP-1 in vitro and in vivo. Int J Mol Med 43:1611-1622 (2019). Read more (PubMed: 30720053) »
