Overview

  • Product name
    Anti-MCP1 antibody
    See all MCP1 primary antibodies
  • Description
    Rabbit polyclonal to MCP1
  • Host species
    Rabbit
  • Specificity
    ab25124 reacts with both rat MCP1 and its mouse homolog JE.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Rat MCP1 aa 1-73 (N terminal).
    Sequence:

    MQVSVTLLGL LFTVAACSIH VLSQPDAVNA PLTCCYSFTG KMIPMSRLEN YKRITSSRCP KEAVVFVTKL KRE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Rat Kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab25124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information).

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Neutralising Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 25 kDa.

Target

  • Function
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • C-C motif chemokine 2 antibody
      • CCL2 antibody
      • CCL2_HUMAN antibody
      • Chemokine (C C motif) ligand 2 antibody
      • GDCF 2 antibody
      • GDCF-2 antibody
      • GDCF2 antibody
      • HC11 antibody
      • HSMCR30 antibody
      • JE antibody
      • MCAF antibody
      • MCP 1 antibody
      • MCP-1 antibody
      • MCP1 antibody
      • MGC9434 antibody
      • Monocyte chemoattractant protein 1 antibody
      • Monocyte chemotactic and activating factor antibody
      • Monocyte chemotactic protein 1 antibody
      • Monocyte secretory protein JE antibody
      • SCYA2 antibody
      • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
      • Small inducible cytokine A2 antibody
      • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
      • Small-inducible cytokine A2 antibody
      • SMC CF antibody
      • SMC-CF antibody
      • SMCCF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (ab25124)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (ab25124)

      IHC image of MCP1 staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25124, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-MCP1 antibody (ab25124)
      Western blot - Anti-MCP1 antibody (ab25124)
      All lanes : Anti-MCP1 antibody (ab25124) at 1/1000 dilution

      Lane 1 : 40ug whole rat spinal cord protein lysate
      Lane 2 : 40ug whole mouse spinal cord protein lysate
      Lane 3 : Naive Rat
      Lane 4 : Sham 1
      Lane 5 : Sham 2
      Lane 6 : Injured 1
      Lane 7 : Injured 2
      Lane 8 : Injured 3

      Secondary
      All lanes : Goat anti-Rabbit HRP

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 2 minutes


      This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 7 December 2005.

      See Abreview

    References

    This product has been referenced in:
    • Zhang H  et al. Renal-protective effect of thalidomide in streptozotocin-induced diabetic rats through anti-inflammatory pathway. Drug Des Devel Ther 12:89-98 (2018). Read more (PubMed: 29386886) »
    • Jadhav K  et al. Reversal of metabolic disorders by pharmacological activation of bile acid receptors TGR5 and FXR. Mol Metab 9:131-140 (2018). WB . Read more (PubMed: 29361497) »

    See all 32 Publications for this product

    Publishing research using ab25124? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    7 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCP1 antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 20 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCP1 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Oct 14 2015

    Western blot abreview for Anti-MCP1 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Recombinant protein (Recombinant protein)
    Loading amount
    0.2 µg
    Specification
    Recombinant protein
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2010

    Can this antibody be used for neutralization (as listed on datasheet) even though it contains azide?

    Thank you for your enquiry. We recommend that the azide be dialyzed out of the antibody preparation before any in vitro or in vivo studies.

    Flow Cytometry abreview for Anti-MCP1 antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Rat Cell (Primary Mixed Cortical Cultures)
    Specification
    Primary Mixed Cortical Cultures
    Permeabilization
    No
    Read More
    abreview image
    Username

    Ms. Nancy Nutile-McMenemy

    Verified customer

    Submitted Apr 12 2006

    Western blot abreview for Anti-MCP1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Spinal Cord)
    Specification
    Spinal Cord
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    abreview image
    Username

    Ms. Nancy Nutile-McMenemy

    Verified customer

    Submitted Dec 07 2005

    I am intrested in detecting MCP-1 in normal and Tg mouse brain cortex by Western Blot analysis. I have used another Ab with little success. Am interested in knowing how much total protein you load to pick up the MCP-1 using the Ab at the recommended 1...

    Read More

    Thank you for your enquiry. This antiserum was tested by western blotting using RAW cells treated with LPS for 15 hours. 10ug of lysate and 10ug of supernatant was loaded onto the gel. A prominent band of 25 kDa was detected. I hope this informat...

    Read More

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