Overview

  • Product name

  • Description

    Rabbit polyclonal to MCP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human MCP1 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab73679)

  • Positive control

    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HCT 116, HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab73680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Images

  • All lanes : Anti-MCP1 antibody (ab73680) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 125 kDa, 50 kDa (possible glycosylated form), 80 kDa. We are unsure as to the identity of these extra bands.



    The expression profile observed is consistent with what has been described in the literature (e.g. PMID 12957789). We believe the 50kDa band is a glycosylated form of Human MCP1.
  • ab73680, at a 1/250 dilution, staining human MCP1 in adrenal gland tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

  • Immunohistochemical analysis of pancreatic ductal adenocarcinoma tissue, staining MCP1 with ab73680.

    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6.0). Tissue was blocked with 1% BSA for 10 min. The samples incubated overnight with primary antibody (1/40). Staining was detected using DAB. Sections were counterstained with hematoxylin.

References

This product has been referenced in:

  • Kobayashi K  et al. Role of monocyte chemoattractant protein-1 in liver fibrosis with transient myeloproliferative disorder in down syndrome. Hepatol Commun 2:230-236 (2018). Read more (PubMed: 29507898) »
  • Zabini D  et al. Comprehensive analysis of inflammatory markers in chronic thromboembolic pulmonary hypertension patients. Eur Respir J 44:951-62 (2014). IHC . Read more (PubMed: 25034560) »
See all 3 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Answer

Thank you for getting back to me and for getting in touch with BioConnect. I will let you know if I don't hear anything from them shortly, and we will figure out the vial of ab9669. I'll also send you a confirmation email after I work everything out with them.

Thank you for your understanding, and let me know if you have any questions in the meantime.

Read More

Answer

Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please keep me updated about the results with this replacement, and let me know if there is anything else that you need!

Read More

Answer

Thank you again for your patience.

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab9669, or ab9858-

https://www.abcam.com/MCP1-antibody-ab9858.html

Please let me know and I will arrange for this to be sent to you promptly. Have a great day!

Read More

Answer

Thank you for your reply.

Please keep me updated on any progress. I hope that we hear something from them soon.

Please let me know if you need anything else from me at this time, and I'll let you know if I hear from someone at BioConnect. You have a good weekend as well!

Read More

Answer

Thank you again for your patience.

At this point, I have not heard from Bio-Connect regarding these issues and I've looked through our system and don't see that they've corresponded with anyone else at Abcam. Have you received any update from them? Is there anyone at Bio-Connect who I can contact?

I look forward to hearing from you. I'll be out of the office Monday-Thursday of next week but I will be back in touch nextFriday if there is anything else that I can do to help resolve these issues. Thanks so much! Have a great weekend.

Read More

Answer

Thank you for your reply and for confirming that information.

I'm processing the refunds and replacements now. I think I've found your order for ab22048, ab9100, and ab8021, but I am having some trouble finding the order for ab9669. Were these ordered through our distributor in the Netherlands, Bio-Connect? If so, could you plrease forward me your shipping address and your Bio-Connect customer or account number, to make sure the refunds and replacements reach you.

For the MCP1 antibody, is it possible that you currently have a different antibody other than ab9669? Our records only show one order of ab9669 shipped to the Netherlands and that was placed in 2008. If you could find out the Abcam order number from your contact at Bio-Connect, it would be very helpful.

I am sorry for the delay in processing these, but I just want to make sure you are properly reimbursed. I look forward to hearing from you, and please let me know if you have any questions or further information. I hope you enjoy your weekend as well!

Read More

Answer

Thank you for getting back to me with your selections.

For CX3CR1, we do have a couple of other anti-human antibodies that have been tested in IHC on FFPE sections, ab8020 and ab51668-

https://www.abcam.com/CX3CR1-antibody-ab8020.html

https://www.abcam.com/CX3CR1-antibody-ab51668.html

Would you be interested in trying one of these as a replacement for ab8021? If not, I will go ahead and proceed with the refund. I will process your requests as soon as I hear back from you!

I look forward to hearing from you. Please let me know if you need anything else, and have a great day!

Read More

Answer

Thank you for getting back to me, and I am happy to help resolve this issue. I look forward to hearing from you, and please let me know if you would like any assistance selecting replacements, or if you have any other questions.

Have a great day!

Read More

Question

Dear sir, madam,

april 2011 our lab ordered the following abcam antibodies

anti-human Cx3Cr1 (ab8021)

anti-human BDNF (ab72439)

anti-human TLR4 (ab22048)

anti-human TLR2 (ab9100)

anti-human MCP-1(ab9669)

anti-human NGF (ab6198)

anti-human TGF-beta (ab66043)

Of these, BDNF, NGF, TGF seem to work fine. However, after using various human tissue imbedded paraffine sections (tonsil, skin psoriasis, lymphnode, and brain sepsis) we still get no (TLR2) and vague/unspecific (MCP1,CX3CR1, TLR4) immunoreactivity (IR).

The most concentrated dilution suggested for these antibodies was used, and antigen retrieval was performed according to protocol (for example TLR4: antigen retrieval with citrate buffer PH6).
The reason I haven't contacted you before, is because I wanted to test the markers on various human tissue which I first had to gather.

Please find images attached in a 'We Tranfer file' of TLR2, TLR4, MCP1 and Cx3Cr1 IHC of cortex sepsis, skin psoriasis, tonsil and lymphnode human paraffine embedded tissue (Images were too big to attach in this email, even when compressed).

I would like your expert opinion on these stainings.
In our opninion
TLR2 shows no immunoreactivity at all (note:TLR2 datasheet explicitly states lymphnode as tissue specificity).
TLR4 shows vessel-staining in lymphnode and cortex, and broader staining in tonsil and psoriasis. However doubtdful on the specificty.
MCP1 shows a diffuse staining pattern, which again may be unspecific?
CX3CR1 shows no immunoreactivity in sepsis cortex nor lymphnode, but some IR in tonsil in psoriasis.

Since these 4 antibodies do not perform as described on the datasheet, I wanted to ask for either a refund or a replacement of these products with Anti-TLR2 (ab24192), Anti-TLR4 (ab13556), Anti-MCP1 (ab73680) and a different batch of the same CxCr1 (ab8021).

Read More
Answer

Thank you for contacting us and letting us know about the trouble with these antibodies.

After reviewing the protocol and images, I agree that these antibodies might not be performing as expected. Our guarantee period generally extends for 6 months after purchase, but I understand that it takes time to thoroughly test so many antibodies, and so I would be happy to send the replacements that you mention or to issue refund. Would prefer a replacement or refund, and could you send me your original order number or PO number?

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal)
Specification
adrenal
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted May 28 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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