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Dear sir, madam,
april 2011 our lab ordered the following abcam antibodies
anti-human Cx3Cr1 (ab8021)
anti-human BDNF (ab72439)
anti-human TLR4 (ab22048)
anti-human TLR2 (ab9100)
anti-human NGF (ab6198)
anti-human TGF-beta (ab66043)
Of these, BDNF, NGF, TGF seem to work fine. However, after using various human tissue imbedded paraffine sections (tonsil, skin psoriasis, lymphnode, and brain sepsis) we still get no (TLR2) and vague/unspecific (MCP1,CX3CR1, TLR4) immunoreactivity (IR).
The most concentrated dilution suggested for these antibodies was used, and antigen retrieval was performed according to protocol (for example TLR4: antigen retrieval with citrate buffer PH6).
The reason I haven't contacted you before, is because I wanted to test the markers on various human tissue which I first had to gather.
Please find images attached in a 'We Tranfer file' of TLR2, TLR4, MCP1 and Cx3Cr1 IHC of cortex sepsis, skin psoriasis, tonsil and lymphnode human paraffine embedded tissue (Images were too big to attach in this email, even when compressed).
I would like your expert opinion on these stainings.
In our opninion
TLR2 shows no immunoreactivity at all (note:TLR2 datasheet explicitly states lymphnode as tissue specificity).
TLR4 shows vessel-staining in lymphnode and cortex, and broader staining in tonsil and psoriasis. However doubtdful on the specificty.
MCP1 shows a diffuse staining pattern, which again may be unspecific?
CX3CR1 shows no immunoreactivity in sepsis cortex nor lymphnode, but some IR in tonsil in psoriasis.
Since these 4 antibodies do not perform as described on the datasheet, I wanted to ask for either a refund or a replacement of these products with Anti-TLR2 (ab24192), Anti-TLR4 (ab13556), Anti-MCP1 (ab73680) and a different batch of the same CxCr1 (ab8021).
Asked on Feb 10 2012
Thank you for contacting us and letting us know about the trouble with these antibodies.
After reviewing the protocol and images, I agree that these antibodies might not be performing as expected. Our guarantee period generally extends for 6 months after purchase, but I understand that it takes time to thoroughly test so many antibodies, and so I would be happy to send the replacements that you mention or to issue refund. Would prefer a replacement or refund, and could you send me your original order number or PO number?
I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.
Answered on Feb 10 2012