Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab7814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration. For neutralization of biological activity in vitro dilutions have to be made according to the amount of MCP-1 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
ELISA Use at an assay dependent concentration. PubMed: 24413421
Inhibition Assay 1/10.
IHC-Fr 1/10.
ICC 1/10.

Target

  • Function
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

References

This product has been referenced in:
  • Cai Z  et al. Transcriptomic analysis of hepatic responses to testosterone deficiency in miniature pigs fed a high-cholesterol diet. BMC Genomics 16:59 (2015). IHC-P ; Pig . Read more (PubMed: 25887406) »
  • Martinez AN  et al. Antagonist of the neurokinin-1 receptor curbs neuroinflammation in ex vivo and in vitro models of Lyme neuroborreliosis. J Neuroinflammation 12:243 (2015). Read more (PubMed: 26714480) »
See all 7 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your message and for providing this further information.

I am sorry to hear that this product has not worked at stated in the datasheet and for the inconvenience this has caused. I appreciate the time you have spent on these experiments, and would like to offer a replacement or refund/credit note in compensation (providing the product has been purchased in the last 180 days). In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Answer

In addition to the information I sent through to you yesterday regarding two alternative antibodies that you may be interested in, I have now received more information from the originator regarding the use of ab7814 in IHC: The following articles feature the use of this antibody to detect MCP-1 on frozen sections: - Expression of Monocyte chemoattractant protein-1 in delayed type hypersensitivity reactions in the skin. Xiaohui Yu et al.; Laboratory investigation, 1992. Vol 71, No. 2, p226. - A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich Elisa and in situ detection of producing cells. Giuseppe Peri et al.

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Answer

I have chased the originator for further information about how ab7814 was used in IHC and will get back to you as soon as I receive their reply. There are two other antibodies against MCP-1 that may be useful for your research: ab7202 -- Rabbit polyclonal to rat MCP 1 / MCAF ab 8102 -- Mouse monoclonal (MNA1) to human MCP 1 / MCAF Please note ab7202 has only been tested for cross reactivity with rat and mouse. Both antibodies are recommended for use in IHC on frozen sections. Please let me know if you would like a complementary vial of either of these antibodies.

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Answer

This antibody has not been tested on formalin fixed paraffin embedded tissues, therefore we can't guarantee results.

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Answer

We have two antibodies to MCP-1: this one which has only been tested on human tissue, and ab7202 which is reactive to both rat and mouse (although again not tested on rabbits). We cannot guarantee that either of these will work on rabbit.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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