Anti-MCP1 antibody (ab9669)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-MCP1 antibody
See all MCP1 primary antibodies -
Description
Rabbit polyclonal to MCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human MCP1.
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Positive control
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General notes
Although some customers have been successful in IHC we no longer batch test in this application. For and IHC validated antibody please see ab73680
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.1 - 0.2 µg/ml.
To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to MCP1 (ab9858).
To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab271250 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
| Notes |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to MCP1 (ab9858). To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab271250 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
Target
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6347 Human
- Omim: 158105 Human
- SwissProt: P13500 Human
- Unigene: 303649 Human
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
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Western blot - Anti-MCP1 antibody (ab9669)
HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 µM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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Sandwich ELISA - Anti-MCP1 antibody (ab9669)Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml. -
Western blot - Anti-MCP1 antibody (ab9669)All lanes : Anti-MCP1 antibody (ab9669) at 0.2 µg/ml
Lane 1 :Recombinant human MCP1 protein (ab73866) at 0.01 µg
Lane 2 :Recombinant human MCP1 protein (ab73866) at 0.001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute -
Western blot - Anti-MCP1 antibody (ab9669)Image courtesy of Dr Laura Toma by Abreview.Anti-MCP1 antibody (ab9669) at 1/4000 dilution + lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 12-14 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (65)
ab9669 has been referenced in 65 publications.
- Sindhu S et al. Enhanced Adipose Expression of Interferon Regulatory Factor (IRF)-5 Associates with the Signatures of Metabolic Inflammation in Diabetic Obese Patients. Cells 9:N/A (2020). PubMed: 32188105
- Zhou DY et al. In silico prediction and validation of potential therapeutic genes in pancreatic ß-cells associated with type 2 diabetes. Exp Ther Med 20:60 (2020). PubMed: 32952650
- Brusletto BS et al. Extensive Changes in Transcriptomic "Fingerprints" and Immunological Cells in the Large Organs of Patients Dying of Acute Septic Shock and Multiple Organ Failure Caused by Neisseria meningitidis. Front Cell Infect Microbiol 10:42 (2020). PubMed: 32154187
- Lin H et al. Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition. Aging (Albany NY) 12:12684-12702 (2020). PubMed: 32629426
- Zhang H et al. Long noncoding RNA MIAT2 alleviates lipopolysaccharide-induced inflammatory damage in WI-38 cells by sponging microRNA-15. J Cell Physiol 235:3690-3697 (2020). PubMed: 31566734