Anti-MCP1 antibody (ab9669)
- Datasheet
- References (33)
- Protocols
Overview
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Product nameAnti-MCP1 antibody
See all MCP1 primary antibodies -
DescriptionRabbit polyclonal to MCP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Sandwich ELISA, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human MCP1.
Sequence:QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
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Positive control
- Purchase matching WB positive control:Recombinant human MCP1 protein
- Recombinant human MCP1 protein (ab9670) can be used as a positive control in WB.
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.40
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab9669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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| Sandwich ELISA | Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to MCP1 (ab9858). To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab9858 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20369226 |
Target
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FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
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Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
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Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 6347 Human
- Omim: 158105 Human
- SwissProt: P13500 Human
- Unigene: 303649 Human
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
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Western blot - Anti-MCP1 antibody (ab9669)HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 µM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (ab9669)ab9669 at 2.5 µg/ml staining human breast invasive ductal carcinoma tissue by IHC-P. The anitbody was incubated for two hours at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. -
Sandwich ELISA - Anti-MCP1 antibody (ab9669)Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml. -
Western blot - Anti-MCP1 antibody (ab9669)All lanes : Anti-MCP1 antibody (ab9669) at 0.2 µg/ml
Lane 1 :Recombinant human MCP1 protein (ab73866) at 0.01 µg
Lane 2 :Recombinant human MCP1 protein (ab73866) at 0.001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute -
Western blot - Anti-MCP1 antibody (ab9669)Image courtesy of Dr Laura Toma by Abreview.Anti-MCP1 antibody (ab9669) at 1/4000 dilution + lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 12-14 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wei CC et al. Allergic Conjunctivitis-induced Retinal Inflammation Promotes Myopia Progression. EBioMedicine 28:274-286 (2018). Read more (PubMed: 29398596) »
- Weiler J et al. Matrix metalloproteinase-9 (MMP9) is involved in the TNF-a-induced fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells. Cell Commun Signal 16:14 (2018). WB . Read more (PubMed: 29636110) »
