Western blot abreview for Anti-MCP1 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Fetal foreskin cells)
Loading amount
10 µg
Specification
Fetal foreskin cells
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST-Milk

Secondary antibody

Name
Non-Abcam antibody was used: LICOR antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000

Detection

Detection method
Odyssey
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 9 kDa Non-specific: 40, 50, 75 kDa
Positive control
human fetal foreskin fibroblasts

Additional data

Additional Notes
Figure Legend: Lane 1: HFFF2- fetal fibroblast lysate Lane 2 and 3: HFFF2 with shRNA specific to CCL2 (absence of ~10Kd band)

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Submitted Feb 17 2011

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