Western blot abreview for Anti-MCP1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (smooth muscle cell-conditioned medium)
Loading amount
10 µg
Specification
smooth muscle cell-conditioned medium
Treatment
100 ug/ml glycated LDL
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/4000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: Tris-Buffered Saline and Tween 20

Secondary antibody

Dilution
1/20000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 12-14 kDa

Additional data

PubMed ID
19818091: View

Dr. Laura Toma

Verified customer

Submitted Feb 24 2011

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