Western blot abreview for Anti-MCP1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Endothelial cells (EA.hy926) - conditioned media)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
500000 cells
Treatment
100 µg/ml glycated LDL (AGE-LDL) for 24 hours
Specification
Endothelial cells (EA.hy926) - conditioned media
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBST plus 5% skim milk
Dilution
1/4000

Secondary antibody

Dilution
1/50000
Name

Detection

Detection method
ECL+
Negative control
None.
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 11-13 kDa
Positive control
None.

Additional data

Additional Notes
No additional bands observed.
PubMed ID
21729693: View

Dr. Anca Sima

Verified customer

Submitted Jun 18 2015

Sign up