- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-MCP1 antibody
See all MCP1 primary antibodies -
DescriptionRabbit polyclonal to MCP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Neutralising, Sandwich ELISA, IHC-FoFrmore details
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Species reactivityReacts with: Rat
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Immunogen
Highly pure (>98%) recombinant rMCAF/MCP-1 (rat Macrophage/Monocyte chemotactic protein-1)
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. sed in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Neutralising | Use a concentration of 4 - 6 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of Rat MCP1 (100 ng/ml). | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP1. | |
| IHC-FoFr | Use at an assay dependent concentration. |
Target
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FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
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Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
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Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 24770 Rat
- SwissProt: P14844 Rat
- Unigene: 4772 Rat
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MCP1 antibody (ab9779)This image is courtesy of the Tissue Profiling group, SciLifeLab StockholmImmunohistochemical analysis of PFA perfusion fixed frozen rat brain, staining MCP1 with ab9779 at 1 µg/ml. A peroxidase conjugated secondary antibody was added and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent.
References
This product has been referenced in:
- Madrigal JL et al. Astrocyte-derived MCP-1 mediates neuroprotective effects of noradrenaline. J Neurosci 29:263-7 (2009). Rat . Read more (PubMed: 19129402) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"