Anti-MCP1 antibody (ab9779)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-MCP1 antibody
See all MCP1 primary antibodies -
Description
Rabbit polyclonal to MCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Neutralising, Sandwich ELISA, IHC-FoFrmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Highly pure (>98%) recombinant rMCAF/MCP-1 (rat Macrophage/Monocyte chemotactic protein-1)
Properties
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Form
Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. sed in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Neutralising | Use a concentration of 4 - 6 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of Rat MCP1 (100 ng/ml). | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP1. | |
| IHC-FoFr | Use at an assay dependent concentration. |
Target
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 24770 Rat
- SwissProt: P14844 Rat
- Unigene: 4772 Rat
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MCP1 antibody (ab9779)This image is courtesy of the Tissue Profiling group, SciLifeLab StockholmImmunohistochemical analysis of PFA perfusion fixed frozen rat brain, staining MCP1 with ab9779 at 1 µg/ml. A peroxidase conjugated secondary antibody was added and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent.
Datasheets and documents
References
This product has been referenced in:
- Zeng J et al. TRIM9-Mediated Resolution of Neuroinflammation Confers Neuroprotection upon Ischemic Stroke in Mice. Cell Rep 27:549-560.e6 (2019). Read more (PubMed: 30970257) »
- Madrigal JL et al. Astrocyte-derived MCP-1 mediates neuroprotective effects of noradrenaline. J Neurosci 29:263-7 (2009). Rat . Read more (PubMed: 19129402) »
