Anti-MCP1 antibody (ab9858)

Reviews (1)Q&A (4)References (3)
Sandwich ELISA - Anti-MCP1 antibody (ab9858)
  • Sandwich ELISA - Anti-MCP1 antibody (ab9858)

Key features and details

  • Mouse monoclonal to MCP1
  • Suitable for: Sandwich ELISA
  • Reacts with: Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-MCP1 antibody
    See all MCP1 primary antibodies

  • Description

    Mouse monoclonal to MCP1

  • Host species

    Mouse

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Recombinant fragment

  • Immunogen

    Recombinant hMCAF/MCP-1 (human Macrophage/Monocyte chemotactic protein-1)

  • Positive control

  • General notes

    This product is no longer batch tested in IHC, for an IHC validated antibody please see ab73680

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669).

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection.
Notes
Sandwich ELISA
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669).

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection.

Target

  • Function

    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.

  • Post-translational
    modifications

    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Images

  • Sandwich ELISA - Anti-MCP1 antibody (ab9858)
    Sandwich ELISA - Anti-MCP1 antibody (ab9858)

    In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 when used with Biotin Anti-MCP1 antibody (ab271250) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

  • Sandwich ELISA - Anti-MCP1 antibody (ab9858)
    Sandwich ELISA - Anti-MCP1 antibody (ab9858)
    Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml.

References (3)

Publishing research using ab9858? Please let us know so that we can cite the reference in this datasheet.

ab9858 has been referenced in 3 publications.

  • Luo S  et al. Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15:7760-7768 (2018). PubMed: 29725470
  • Ji WT  et al. Monocyte chemotactic protein 1 (MCP-1) modulates pro-survival signaling to promote progression of head and neck squamous cell carcinoma. PLoS One 9:e88952 (2014). IHC-P ; Human . PubMed: 24586454
  • Tripathy D  et al. Thrombin, a mediator of cerebrovascular inflammation in AD and hypoxia. Front Aging Neurosci 5:19 (2013). IHC-P ; Mouse . PubMed: 23675346

Customer reviews and Q&As

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Western blot abreview for Anti-MCP1 antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% Gel)
Sample
Human Tissue lysate - whole (adipose tissue)
Specification
adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: 20°C
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Mr. Ashley Davies

Verified customer

Submitted Feb 03 2014

Question


We have recieved the Ab ab9858 reconstituted in about 500 µl (to be confirmed).
In your spec sheet you mentione that the Ab should be lyophylized. Could you
confirm this? Is there a problem on the sheet or the Ab?
Thank you.
Cheers,

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Answer

Thank you for your recent enquiry.

I have confirmed with our goods out department that this item was reconstituted before sending out to you.

Therefore, you will not need to do any further reconstitution, and I can suggest to ensure it is stored as stated on the datasheet:

Store at +4oC short term (1-2 weeks). Aliquot and store at -20oC long term. Avoid repeated freeze / thaw cycles.

I sincerely apologize for any confusion in this case. I would like to reassure you that the antibody will be covered by our 6 month Abpromise guarantee for the tested applications and species listed on the datasheet.

I hope this will be helpful to you. Please do not hesitate to contact me if you have any further questions or concerns.

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Question

\My lab is trying to setup a IHC protocol for MCP-1 staining in human kidney tissue.
Could youprovided meinformation about staining protocol and test result of theAnti-MCP-1 antibody (ab9858)

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Answer

Thank you for contacting Abcam.

All of the IHC information that we have for ab9858 is available on the website, below is a copy of that protocol information:

"ab9858 staining MCP1 in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 10 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen"

I have also attached the Abcam general IHC protocol for you as well.

Please let me know if there is anything else I can help you with.

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Question

Thank you for your kind support. We received an inquire about #ab9858 from our customer who interested in it. They would like to know clone No. of this product if there is it. Please let us know it. Thanks and sincerely, Best Regards,

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Answer

Thank you for your inquiry about the clone number for ab9858. I'm really sorry about the delay in giving you this information, I had to contact the source. We currently hold 078Y023 for ab9858 therefore, the clone number is PEPY023_hMCP1. I hope this information helps,

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Question

Can you please tell me what the clone is for this product? Thank you.

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Answer

There is no known clone number for this antibody

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