Anti-MCP1 antibody (ab9858)

Reviews (1)Q&A (4)References (3)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669).

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.2 - 0.4 µg/ml.

Target

  • Function

    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.

  • Post-translational
    modifications

    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • C-C motif chemokine 2 antibody
      • CCL2 antibody
      • CCL2_HUMAN antibody
      • Chemokine (C C motif) ligand 2 antibody
      • GDCF 2 antibody
      • GDCF-2 antibody
      • GDCF2 antibody
      • HC11 antibody
      • HSMCR30 antibody
      • JE antibody
      • MCAF antibody
      • MCP 1 antibody
      • MCP-1 antibody
      • MCP1 antibody
      • MGC9434 antibody
      • Monocyte chemoattractant protein 1 antibody
      • Monocyte chemotactic and activating factor antibody
      • Monocyte chemotactic protein 1 antibody
      • Monocyte secretory protein JE antibody
      • SCYA2 antibody
      • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
      • Small inducible cytokine A2 antibody
      • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
      • Small-inducible cytokine A2 antibody
      • SMC CF antibody
      • SMC-CF antibody
      • SMCCF antibody
      see all

    Images

    • Sandwich ELISA - Anti-MCP1 antibody (ab9858)
      Sandwich ELISA - Anti-MCP1 antibody (ab9858)
      Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (ab9858)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (ab9858)

      ab9858 staining MCP1 in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 10 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

    References

    This product has been referenced in:

    • Luo S  et al. Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15:7760-7768 (2018). Read more (PubMed: 29725470) »
    • Ji WT  et al. Monocyte chemotactic protein 1 (MCP-1) modulates pro-survival signaling to promote progression of head and neck squamous cell carcinoma. PLoS One 9:e88952 (2014). IHC-P ; Human . Read more (PubMed: 24586454) »
    See all 3 Publications for this product

    Publishing research using ab9858? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-MCP1 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10% Gel)
    Sample
    Human Tissue lysate - whole (adipose tissue)
    Specification
    adipose tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Mr. Ashley Davies

    Verified customer

    Submitted Feb 03 2014

    Question


    We have recieved the Ab ab9858 reconstituted in about 500 µl (to be confirmed).
    In your spec sheet you mentione that the Ab should be lyophylized. Could you
    confirm this? Is there a problem on the sheet or the Ab?
    Thank you.
    Cheers,

    Read More
    Answer

    Thank you for your recent enquiry.

    I have confirmed with our goods out department that this item was reconstituted before sending out to you.

    Therefore, you will not need to do any further reconstitution, and I can suggest to ensure it is stored as stated on the datasheet:

    Store at +4oC short term (1-2 weeks). Aliquot and store at -20oC long term. Avoid repeated freeze / thaw cycles.

    I sincerely apologize for any confusion in this case. I would like to reassure you that the antibody will be covered by our 6 month Abpromise guarantee for the tested applications and species listed on the datasheet.

    I hope this will be helpful to you. Please do not hesitate to contact me if you have any further questions or concerns.

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    Question

    \My lab is trying to setup a IHC protocol for MCP-1 staining in human kidney tissue.
    Could youprovided meinformation about staining protocol and test result of theAnti-MCP-1 antibody (ab9858)

    Read More
    Answer

    Thank you for contacting Abcam.

    All of the IHC information that we have for ab9858 is available on the website, below is a copy of that protocol information:

    "ab9858 staining MCP1 in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 10 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen"

    I have also attached the Abcam general IHC protocol for you as well.

    Please let me know if there is anything else I can help you with.

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    Question

    Thank you for your kind support. We received an inquire about #ab9858 from our customer who interested in it. They would like to know clone No. of this product if there is it. Please let us know it. Thanks and sincerely, Best Regards,

    Read More
    Answer

    Thank you for your inquiry about the clone number for ab9858. I'm really sorry about the delay in giving you this information, I had to contact the source. We currently hold 078Y023 for ab9858 therefore, the clone number is PEPY023_hMCP1. I hope this information helps,

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    Question

    Can you please tell me what the clone is for this product? Thank you.

    Read More
    Answer

    There is no known clone number for this antibody

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