FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
- Information by UniProt
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
Western blot analysis of varying levels of Recombinant human MCP-1 protein using ab271250 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Human MCP1 was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271250. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant MCP1 protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271250 has not yet been referenced specifically in any publications.