Anti-MCP1 antibody [ECE.2] (ab8101)

Thank you for your reply.

I can confirm this antibody ab8101 is not conjugated. If an antibody is conjugated, this information is provided on the datasheet.

I can recommend to consider conjugating the antibody with a conjugate of your choice from our Easy Link conjugation kit range:

For more information, please view the following page from our website. There a link to lists of our Easylink products at the top of this page:

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

I can also recommend to read the frequently asked questions page carefully for more information:

https://www.abcam.com/index.html?pageconfig=resource&rid=13156

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

Thank you for contacting us. Yes, this antibody has been tested and guaranteed for use in IHC. Please see the references for images.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

For WB,here's the protocol used:

Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 µg/ml) for 1 hr at RT.

Your protocol seems like it should have worked as it was pretty similar.

How much protein was loaded per lane?

What was the species/cell line tested?

This clone was used in WB in this reference: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 9176841

Probably the best positive control would just be an recombinant mouse MCP1 protein, like ab9901.

https://www.abcam.com/JE-protein-Mouse-ab9901.html

Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for your enquiry. For a positive tissue control for ab8101, we recommend any vascular tissue, for instance spleen or lung. For antigen retrieval, we do not have information on which AR treatment, if any, is optimal for this antibody. We suggest trying a heat-mediated citrate pH6.0 AR, as described in the Protocols section of the datasheet: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=8101&mode=problems You may also want to try a "+/- AR" test as the epitopes some monoclonal antibodies bind are sensitive to heat or protease treatment and may not require any AR for good staining. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. We're currently aware of one publication which featured the use of this antibody: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 9176841 We don't have a specific protocol for this antibody, and would recommend referring to our general protocols which can be found by clicking on the protocols tab on the online product datasheet. Please contact us again if you have any additional questions.

Thank you for your question regarding ab8101. The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

The antibody reacts with murine JE / MCP-1, but it has not yet been tested for use with paraffin embedded tissue.