Anti-MCP1 antibody [ECE.2] (ab8101)

Reviews (1)Q&A (8)References (16)

Overview

  • Product name
    Anti-MCP1 antibody [ECE.2]
    See all MCP1 primary antibodies
  • Description
    Rat monoclonal [ECE.2] to MCP1
  • Host species
    Rat
  • Specificity
    The antibody reacts with murine JE / MCP-1.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Synthetic peptide:

    LFKTASALRSSAPLNVKLTRKSEANASTT

    , corresponding to C terminal amino acids 102-130 of Mouse MCP1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Recombinant mouse MCP1 protein (ab9901) can be used as a positive control in WB.
  • General notes
    Monocyte chemotactic protein-1 (MCP-1) is also known as MCAF.

Properties

Applications

Our Abpromise guarantee covers the use of ab8101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150. PubMed: 15829914
IHC-Fr Use at an assay dependent dilution. PubMed: 19920349
IHC-P 1/50.
WB 1/50.

Target

  • Function
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • C-C motif chemokine 2 antibody
      • CCL2 antibody
      • CCL2_HUMAN antibody
      • Chemokine (C C motif) ligand 2 antibody
      • GDCF 2 antibody
      • GDCF-2 antibody
      • GDCF2 antibody
      • HC11 antibody
      • HSMCR30 antibody
      • JE antibody
      • MCAF antibody
      • MCP 1 antibody
      • MCP-1 antibody
      • MCP1 antibody
      • MGC9434 antibody
      • Monocyte chemoattractant protein 1 antibody
      • Monocyte chemotactic and activating factor antibody
      • Monocyte chemotactic protein 1 antibody
      • Monocyte secretory protein JE antibody
      • SCYA2 antibody
      • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
      • Small inducible cytokine A2 antibody
      • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
      • Small-inducible cytokine A2 antibody
      • SMC CF antibody
      • SMC-CF antibody
      • SMCCF antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-MCP1 antibody [ECE.2] (ab8101)
      Immunohistochemistry (Frozen sections) - Anti-MCP1 antibody [ECE.2] (ab8101)

      Detection of MCP-1 in mouse testis. ab8101 has been used in a 10x dilution, the magnification was 200x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [ECE.2] (ab8101)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [ECE.2] (ab8101)Image from Lau KS et al., PLoS Biol. 2012;10(9):e1001393. doi: 10.1371/journal.pbio.1001393. Epub 2012 Sep 25.; Fig 5.; September 25, 2012, PLoS Biol 10(9): e1001393.
      Immunohistochemical analysis of formalin-fixed murine duodenal tissue, staining MCP1 with ab8101.

    References

    This product has been referenced in:
    • Brun P  et al. Herpes Simplex Virus Type 1 Infects Enteric Neurons and Triggers Gut Dysfunction via Macrophage Recruitment. Front Cell Infect Microbiol 8:74 (2018). IHC-P . Read more (PubMed: 29600197) »
    • Hsu PL  et al. Ganoderma Triterpenoids Exert Antiatherogenic Effects in Mice by Alleviating Disturbed Flow-Induced Oxidative Stress and Inflammation. Oxid Med Cell Longev 2018:3491703 (2018). Read more (PubMed: 29849882) »
    See all 16 Publications for this product

    Publishing research using ab8101? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCP1 antibody [ECE.2]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Adipose near brain tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Adipose near brain tumor
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 02 2018

    Question

    I would like to order MCP1 protein (Active) (ab9901) and Anti-MCP1 antibody [ECE.2] (ab8101). However, it would really help my experiment if antibody had a fluorescent tag attached to it. I can’t tell from the information on the site if this is the case.

    Read More
    Answer

    Thank you for your reply.

    I can confirm this antibody ab8101 is not conjugated. If an antibody is conjugated, this information is provided on the datasheet.

    I can recommend to consider conjugating the antibody with a conjugate of your choice from our Easy Link conjugation kit range:

    For more information, please view the following page from our website. There a link to lists of our Easylink products at the top of this page:

    https://www.abcam.com/index.html?pageconfig=resource&rid=13148

    I can also recommend to read the frequently asked questions page carefully for more information:

    https://www.abcam.com/index.html?pageconfig=resource&rid=13156

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

    Read More

    Question



    I see online it can be used IHC, but there is no picture. Has this been validated and can you provide a picture?
    Thanks

    Read More
    Answer

    Thank you for contacting us. Yes, this antibody has been tested and guaranteed for use in IHC. Please see the references for images.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    Greetings,
    I recently ordered and received this antibody from you all. I am writing
    to request some technical assistance as I was unable to detect the protein
    of interest in cell extracts which had been shown by ELISA to secrete
    large amounts of MCP-1.
    My questions are
    1. What is the best protocol for western blotting using this antibody?
    2. What is the best positive control for its expression.
    In summary, our cell extracts were produced by lysis in RIPA buffer
    supplemented with protease inhibitors. After protein quantitation, a large
    of amount of protein was loaded onto an 8-15% gradient SDS-PAGE, then
    transferred to PVDF membrane. The protein transfer verified by Ponceau S
    staining. I blocked in 5% Milk/TBST for 1 h, room temp. incubated the
    membrane in ab8108 at 1:50 in 1% Milk/TBST overnight at 4C. Secondary was
    anti-Rat-HRP at 1:1000 in 1% Milk/TBST for 1 h. We used regular ECL for
    detection.

    Read More
    Answer

    Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    For WB,here's the protocol used:

    Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 µg/ml) for 1 hr at RT.

    Your protocol seems like it should have worked as it was pretty similar.

    How much protein was loaded per lane?

    What was the species/cell line tested?

    This clone was used in WB in this reference: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 9176841

    Probably the best positive control would just be an recombinant mouse MCP1 protein, like ab9901.

    https://www.abcam.com/JE-protein-Mouse-ab9901.html

    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Question

    Which mouse tissue is suitable as positive control ? is any pre-treatment (e.g. protease, microwave) necessary?

    Read More
    Answer

    Thank you for your enquiry. For a positive tissue control for ab8101, we recommend any vascular tissue, for instance spleen or lung. For antigen retrieval, we do not have information on which AR treatment, if any, is optimal for this antibody. We suggest trying a heat-mediated citrate pH6.0 AR, as described in the Protocols section of the datasheet: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=8101&mode=problems You may also want to try a "+/- AR" test as the epitopes some monoclonal antibodies bind are sensitive to heat or protease treatment and may not require any AR for good staining. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Are there any published protocols using this ECE.2 antibody?

    Read More
    Answer

    Thank you for your enquiry. We're currently aware of one publication which featured the use of this antibody: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 9176841 We don't have a specific protocol for this antibody, and would recommend referring to our general protocols which can be found by clicking on the protocols tab on the online product datasheet. Please contact us again if you have any additional questions.

    Read More

    Question

    I would like to know about the molecular weight of MCP-1 using Rat monoclonal to MCP-1(ab8101) in Western blotting.

    Read More
    Answer

    Thank you for your question regarding ab8101. The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

    Read More

    Question

    Customer's question: I would like to know about the molecular weight of MCP-1 using Rat monoclonal to MCP-1(ab8101) in Western blotting.

    Read More
    Answer

    The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

    Read More

    Question

    Does this antibody work on mouse paraffin embedded tissues samples?

    Read More
    Answer

    The antibody reacts with murine JE / MCP-1, but it has not yet been tested for use with paraffin embedded tissue.

    Read More

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