Anti-MCP1 antibody [ECE.2] (ab8101)

Reviews (1)Q&A (8)References (22)
Immunohistochemistry (Frozen sections) - Anti-MCP1 antibody [ECE.2] (ab8101)

    Key features and details

    • Rat monoclonal [ECE.2] to MCP1
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG1

    Overview

    • Product name

      Anti-MCP1 antibody [ECE.2]
      See all MCP1 primary antibodies

    • Description

      Rat monoclonal [ECE.2] to MCP1

    • Host species

      Rat

    • Specificity

      The antibody reacts with murine JE / MCP-1.

    • Tested Applications & Species

      Application Species
      IHC-Fr
      Mouse
      See all applications and species data

    • Immunogen

      Synthetic peptide:

      LFKTASALRSSAPLNVKLTRKSEANASTT

      , corresponding to C terminal amino acids 102-130 of Mouse MCP1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Recombinant mouse MCP1 protein (ab9901) can be used as a positive control in WB.

    • General notes

      Monocyte chemotactic protein-1 (MCP-1) is also known as MCAF.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab8101 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-Fr
    Mouse
    Application Abreviews Notes
    IHC-Fr
    Use at an assay dependent concentration.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.

    Target

    • Function

      Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.

    • Post-translational
      modifications

      Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • C-C motif chemokine 2 antibody
        • CCL2 antibody
        • CCL2_HUMAN antibody
        • Chemokine (C C motif) ligand 2 antibody
        • GDCF 2 antibody
        • GDCF-2 antibody
        • GDCF2 antibody
        • HC11 antibody
        • HSMCR30 antibody
        • JE antibody
        • MCAF antibody
        • MCP 1 antibody
        • MCP-1 antibody
        • MCP1 antibody
        • MGC9434 antibody
        • Monocyte chemoattractant protein 1 antibody
        • Monocyte chemotactic and activating factor antibody
        • Monocyte chemotactic protein 1 antibody
        • Monocyte secretory protein JE antibody
        • SCYA2 antibody
        • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
        • Small inducible cytokine A2 antibody
        • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
        • Small-inducible cytokine A2 antibody
        • SMC CF antibody
        • SMC-CF antibody
        • SMCCF antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-MCP1 antibody [ECE.2] (ab8101)
        Immunohistochemistry (Frozen sections) - Anti-MCP1 antibody [ECE.2] (ab8101)

        Detection of MCP-1 in mouse testis. ab8101 has been used in a 10x dilution, the magnification was 200x.

      References (22)

      Publishing research using ab8101? Please let us know so that we can cite the reference in this datasheet.

      ab8101 has been referenced in 22 publications.

      • Voss S  et al. Macrophage Migration Inhibitory Factor (MIF) Expression Increases during Myocardial Infarction and Supports Pro-Inflammatory Signaling in Cardiac Fibroblasts. Biomolecules 9:N/A (2019). PubMed: 30678084
      • Yang C  et al. Thyrotropin aggravates atherosclerosis by promoting macrophage inflammation in plaques. J Exp Med 216:1182-1198 (2019). PubMed: 30940720
      • Deng B  et al. Progressive Degeneration and Inhibition of Peripheral Nerve Regeneration in the SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis. Cell Physiol Biochem 46:2358-2372 (2018). PubMed: 29742495
      • Curry ZA  et al. Monoacylglycerol Lipase Inhibitors Reverse Paclitaxel-Induced Nociceptive Behavior and Proinflammatory Markers in a Mouse Model of Chemotherapy-Induced Neuropathy. J Pharmacol Exp Ther 366:169-183 (2018). PubMed: 29540562
      • Brun P  et al. Herpes Simplex Virus Type 1 Infects Enteric Neurons and Triggers Gut Dysfunction via Macrophage Recruitment. Front Cell Infect Microbiol 8:74 (2018). IHC-P . PubMed: 29600197
      View all Publications for this product

      Customer reviews and Q&As

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      1-9 of 9 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCP1 antibody [ECE.2]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Adipose near brain tumor)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
      Permeabilization
      No
      Specification
      Adipose near brain tumor
      Blocking step
      PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 02 2018

      Question

      I would like to order MCP1 protein (Active) (ab9901) and Anti-MCP1 antibody [ECE.2] (ab8101). However, it would really help my experiment if antibody had a fluorescent tag attached to it. I can’t tell from the information on the site if this is the case.

      Read More
      Answer

      Thank you for your reply.

      I can confirm this antibody ab8101 is not conjugated. If an antibody is conjugated, this information is provided on the datasheet.

      I can recommend to consider conjugating the antibody with a conjugate of your choice from our Easy Link conjugation kit range:

      For more information, please view the following page from our website. There a link to lists of our Easylink products at the top of this page:

      https://www.abcam.com/index.html?pageconfig=resource&rid=13148

      I can also recommend to read the frequently asked questions page carefully for more information:

      https://www.abcam.com/index.html?pageconfig=resource&rid=13156

      I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

      Read More

      Question



      I see online it can be used IHC, but there is no picture. Has this been validated and can you provide a picture?
      Thanks

      Read More
      Answer

      Thank you for contacting us. Yes, this antibody has been tested and guaranteed for use in IHC. Please see the references for images.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      Greetings,
      I recently ordered and received this antibody from you all. I am writing
      to request some technical assistance as I was unable to detect the protein
      of interest in cell extracts which had been shown by ELISA to secrete
      large amounts of MCP-1.
      My questions are
      1. What is the best protocol for western blotting using this antibody?
      2. What is the best positive control for its expression.
      In summary, our cell extracts were produced by lysis in RIPA buffer
      supplemented with protease inhibitors. After protein quantitation, a large
      of amount of protein was loaded onto an 8-15% gradient SDS-PAGE, then
      transferred to PVDF membrane. The protein transfer verified by Ponceau S
      staining. I blocked in 5% Milk/TBST for 1 h, room temp. incubated the
      membrane in ab8108 at 1:50 in 1% Milk/TBST overnight at 4C. Secondary was
      anti-Rat-HRP at 1:1000 in 1% Milk/TBST for 1 h. We used regular ECL for
      detection.

      Read More
      Answer

      Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

      For WB,here's the protocol used:

      Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 µg/ml) for 1 hr at RT.

      Your protocol seems like it should have worked as it was pretty similar.

      How much protein was loaded per lane?

      What was the species/cell line tested?

      This clone was used in WB in this reference: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 9176841

      Probably the best positive control would just be an recombinant mouse MCP1 protein, like ab9901.

      https://www.abcam.com/JE-protein-Mouse-ab9901.html

      Should the suggestions not improve the results, please do let me know.

      In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

      I hope this information is helpful, and I thank you for your cooperation.

      Read More

      Question

      Which mouse tissue is suitable as positive control ? is any pre-treatment (e.g. protease, microwave) necessary?

      Read More
      Answer

      Thank you for your enquiry. For a positive tissue control for ab8101, we recommend any vascular tissue, for instance spleen or lung. For antigen retrieval, we do not have information on which AR treatment, if any, is optimal for this antibody. We suggest trying a heat-mediated citrate pH6.0 AR, as described in the Protocols section of the datasheet: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=8101&mode=problems You may also want to try a "+/- AR" test as the epitopes some monoclonal antibodies bind are sensitive to heat or protease treatment and may not require any AR for good staining. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Are there any published protocols using this ECE.2 antibody?

      Read More
      Answer

      Thank you for your enquiry. We're currently aware of one publication which featured the use of this antibody: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 9176841 We don't have a specific protocol for this antibody, and would recommend referring to our general protocols which can be found by clicking on the protocols tab on the online product datasheet. Please contact us again if you have any additional questions.

      Read More

      Question

      I would like to know about the molecular weight of MCP-1 using Rat monoclonal to MCP-1(ab8101) in Western blotting.

      Read More
      Answer

      Thank you for your question regarding ab8101. The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

      Read More

      Question

      Customer's question: I would like to know about the molecular weight of MCP-1 using Rat monoclonal to MCP-1(ab8101) in Western blotting.

      Read More
      Answer

      The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

      Read More

      Question

      Does this antibody work on mouse paraffin embedded tissues samples?

      Read More
      Answer

      The antibody reacts with murine JE / MCP-1, but it has not yet been tested for use with paraffin embedded tissue.

      Read More

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