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    mcp1-antibody-ep1361-bsa-and-azide-free-ab240120.pdf

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RecombinantRabMAb

Recombinant Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)

  • Datasheet
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Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
  • Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
  • Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
  • Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1361] to MCP1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant human MCP1 protein (ab73866)

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Overview

  • Product name

    Anti-MCP1 antibody [EP1361] - BSA and Azide free
    See all MCP1 primary antibodies
  • Description

    Rabbit monoclonal [EP1361] to MCP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MCP1 aa 50 to the C-terminus. The exact sequence is proprietary.

  • General notes

    Ab240120 is the carrier-free version of ab151538. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1361
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-MCP1 antibody [EP1361] (ab151538)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human MCP1 protein (ab73866)
  • Related Products

    • Recombinant human MCP1 protein (ab73866)

Applications

Our Abpromise guarantee covers the use of ab240120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications

      Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P13500 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6347 Human
      • Omim: 158105 Human
      • SwissProt: P13500 Human
      • Unigene: 303649 Human
      • Alternative names

        • C-C motif chemokine 2 antibody
        • CCL2 antibody
        • CCL2_HUMAN antibody
        • Chemokine (C C motif) ligand 2 antibody
        • GDCF 2 antibody
        • GDCF-2 antibody
        • GDCF2 antibody
        • HC11 antibody
        • HSMCR30 antibody
        • JE antibody
        • MCAF antibody
        • MCP 1 antibody
        • MCP-1 antibody
        • MCP1 antibody
        • MGC9434 antibody
        • Monocyte chemoattractant protein 1 antibody
        • Monocyte chemotactic and activating factor antibody
        • Monocyte chemotactic protein 1 antibody
        • Monocyte secretory protein JE antibody
        • SCYA2 antibody
        • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
        • Small inducible cytokine A2 antibody
        • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
        • Small-inducible cytokine A2 antibody
        • SMC CF antibody
        • SMC-CF antibody
        • SMCCF antibody
        see all

      Images

      • Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
        Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
        Anti-MCP1 antibody [EP1361] (ab151538) at 1/5000 dilution (purified) + MCP-1 recombinant protein at 0.01 µg

        Secondary
        Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Predicted band size: 11 kDa
        Observed band size: 11 kDa



        This data was developed using ab151538, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
        Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
        Anti-MCP1 antibody [EP1361] (ab151538) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Predicted band size: 11 kDa
        Observed band size: 30 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab151538, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
        Western blot - Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
        All lanes : Anti-MCP1 antibody [EP1361] (ab151538) at 1/1000 dilution (unpurified)

        Lane 1 : Human fetal brain tissue lysate
        Lane 2 : Recombinant human MCP1 (76aa, 8.6kDa)

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 11 kDa



        This data was developed using ab151538, the same antibody clone in a different buffer formulation.

         

      • Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)
        Anti-MCP1 antibody [EP1361] - BSA and Azide free (ab240120)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab240120? Please let us know so that we can cite the reference in this datasheet.

      ab240120 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab240120.
      Please use the links above to contact us or submit feedback about this product.

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